CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036439
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic 
Protein Synonyms/Alias
  
Gene Name
 Gpd1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
49EEDIGGRKLTEIINTubiquitination[1]
62NTQHENVKYLPGHKLubiquitination[1]
83GKICDQLKGHLKANTacetylation[2]
83GKICDQLKGHLKANTubiquitination[1]
87DQLKGHLKANTIGISubiquitination[1]
97TIGISLIKGVDEGPNubiquitination[1]
107DEGPNGLKLISEVIGacetylation[2]
107DEGPNGLKLISEVIGubiquitination[1]
146CETTIGCKDPAQGQLubiquitination[1]
155PAQGQLLKDLMQTPNubiquitination[1]
249CYGGRNRKVAEAFARubiquitination[1]
259EAFARTGKSIEQLEKacetylation[3]
266KSIEQLEKEMLNGQKacetylation[3, 4]
266KSIEQLEKEMLNGQKsuccinylation[4]
266KSIEQLEKEMLNGQKubiquitination[1]
273KEMLNGQKLQGPQTAacetylation[3]
273KEMLNGQKLQGPQTAubiquitination[1]
290LHSILQHKGLVDKFPubiquitination[1]
295QHKGLVDKFPLFTAVacetylation[2]
295QHKGLVDKFPLFTAVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 326 AA 
Protein Sequence
MAGKKVCIVG SGNWGSAIAK IVGSNAGRLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN 60
VKYLPGHKLP PNVFIGKICD QLKGHLKANT IGISLIKGVD EGPNGLKLIS EVIGERLGIP 120
MSVLMGANIA SEVAEEKFCE TTIGCKDPAQ GQLLKDLMQT PNFRITVVQE VDTVEICGAL 180
KNIVAVGAGF CDGLGFGDNT KAAVIRLGLM EMIAFAKLFC SGTVSSATFL ESCGVADLIT 240
TCYGGRNRKV AEAFARTGKS IEQLEKEMLN GQKLQGPQTA RELHSILQHK GLVDKFPLFT 300
AVYKVCYEGQ PVGEFIRCLQ NHPEHM 326 
Gene Ontology
 GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; TAS:MGI.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IEA:InterPro.
 GO:0004368; F:glycerol-3-phosphate dehydrogenase activity; IDA:MGI.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0071320; P:cellular response to cAMP; IDA:MGI.
 GO:0071356; P:cellular response to tumor necrosis factor; IDA:MGI.
 GO:0006094; P:gluconeogenesis; IMP:MGI.
 GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
 GO:0006072; P:glycerol-3-phosphate metabolic process; IMP:MGI. 
Interpro
 IPR008927; 6-PGluconate_DH_C-like.
 IPR013328; DH_multihelical.
 IPR006168; G3P_DH_NAD-dep.
 IPR006109; G3P_DH_NAD-dep_C.
 IPR017751; G3P_DH_NAD-dep_euk.
 IPR011128; G3P_DH_NAD-dep_N.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF07479; NAD_Gly3P_dh_C
 PF01210; NAD_Gly3P_dh_N 
SMART
  
PROSITE
 PS00957; NAD_G3PDH 
PRINTS
 PR00077; GPDHDRGNASE.