CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042873
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Tripartite motif-containing protein 65 
Protein Synonyms/Alias
  
Gene Name
 TRIM65 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
80LRSIEVAKTQALAQAubiquitination[1, 2, 3, 4, 5, 6]
173SHPGAPAKPVDLAPVubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 288 AA 
Protein Sequence
XALLDAERLK REAQLRASLE VTQQQATQAE GQLLELRKQS SQIQNSACIL ASWVSGKFSS 60
LLQALEIQHT TALRSIEVAK TQALAQARDE EQRLRVHLEA VARHGCRIRE LLEQVDEQTF 120
LQESQLLQPP GPLGPLTPLQ WDEDQQLGDL KQLLSRLCGL LLEEGSHPGA PAKPVDLAPV 180
DYRNLTFDPV SANRHFYLSR QDQQVKHCRQ SRGPGGPGSF ELWQVQCAQS FQAGHHYWEV 240
RASDHSVTLG VSYPQLPRCR LGPHTDNIGR GPCSWGLCVQ EDSLQAWH 288 
Gene Ontology
  
Interpro
 IPR001870; B30.2/SPRY.
 IPR008985; ConA-like_lec_gl_sf. 
Pfam
  
SMART
  
PROSITE
 PS50188; B302_SPRY 
PRINTS