CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016208
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinectin 
Protein Synonyms/Alias
 CG-1 antigen; Kinesin receptor 
Gene Name
 KTN1 
Gene Synonyms/Alias
 CG1; KIAA0004 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
248DSSPVVDKREVIDLLubiquitination[1]
256REVIDLLKPDQVEGIubiquitination[1, 2, 3, 4]
265DQVEGIQKSGTKKLKacetylation[5]
269GIQKSGTKKLKTETDacetylation[5]
270IQKSGTKKLKTETDKacetylation[5]
285ENAEVKFKDFLLSLKubiquitination[6, 7, 8]
292KDFLLSLKTMMFSEDphosphoglycerylation[9]
309LCVVDLLKEKSGVIQubiquitination[8, 10]
443TTNQLESKQSAELNKubiquitination[7]
543LTDTLVSKQQLEQRLubiquitination[7]
777TENSSLTKEVQDLKAubiquitination[7]
842KQEIKALKEEIGNVQubiquitination[3]
1296SLELIQSKIVKAAGDubiquitination[6, 8]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [10] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265
Functional Description
 Receptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin. 
Sequence Annotation
 MOD_RES 75 75 Phosphoserine.
 MOD_RES 153 153 Phosphothreonine.
 MOD_RES 156 156 Phosphoserine.
 CARBOHYD 172 172 N-linked (GlcNAc...) (Potential).
 CARBOHYD 435 435 N-linked (GlcNAc...) (Potential).
 CARBOHYD 772 772 N-linked (GlcNAc...) (Potential).
 CARBOHYD 904 904 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1055 1055 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1088 1088 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1263 1263 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1329 1329 N-linked (GlcNAc...) (Potential).
 CROSSLNK 309 309 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Isopeptide bond; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1357 AA 
Protein Sequence
MEFYESAYFI VLIPSIVITV IFLFFWLFMK ETLYDEVLAK QKREQKLIPT KTDKKKAEKK 60
KNKKKEIQNG NLHESDSESV PRDFKLSDAL AVEDDQVAPV PLNVVETSSS VRERKKKEKK 120
QKPVLEEQVI KESDASKIPG KKVEPVPVTK QPTPPSEAAA SKKKPGQKKS KNGSDDQDKK 180
VETLMVPSKR QEALPLHQET KQESGSGKKK ASSKKQKTEN VFVDEPLIHA TTYIPLMDNA 240
DSSPVVDKRE VIDLLKPDQV EGIQKSGTKK LKTETDKENA EVKFKDFLLS LKTMMFSEDE 300
ALCVVDLLKE KSGVIQDALK KSSKGELTTL IHQLQEKDKL LAAVKEDAAA TKDRCKQLTQ 360
EMMTEKERSN VVITRMKDRI GTLEKEHNVF QNKIHVSYQE TQQMQMKFQQ VREQMEAEIA 420
HLKQENGILR DAVSNTTNQL ESKQSAELNK LRQDYARLVN ELTEKTGKLQ QEEVQKKNAE 480
QAATQLKVQL QEAERRWEEV QSYIRKRTAE HEAAQQDLQS KFVAKENEVQ SLHSKLTDTL 540
VSKQQLEQRL MQLMESEQKR VNKEESLQMQ VQDILEQNEA LKAQIQQFHS QIAAQTSASV 600
LAEELHKVIA EKDKQIKQTE DSLASERDRL TSKEEELKDI QNMNFLLKAE VQKLQALANE 660
QAAAAHELEK MQQSVYVKDD KIRLLEEQLQ HEISNKMEEF KILNDQNKAL KSEVQKLQTL 720
VSEQPNKDVV EQMEKCIQEK DEKLKTVEEL LETGLIQVAT KEEELNAIRT ENSSLTKEVQ 780
DLKAKQNDQV SFASLVEELK KVIHEKDGKI KSVEELLEAE LLKVANKEKT VQDLKQEIKA 840
LKEEIGNVQL EKAQQLSITS KVQELQNLLK GKEEQMNTMK AVLEEKEKDL ANTGKWLQDL 900
QEENESLKAH VQEVAQHNLK EASSASQFEE LEIVLKEKEN ELKRLEAMLK ERESDLSSKT 960
QLLQDVQDEN KLFKSQIEQL KQQNYQQASS FPPHEELLKV ISEREKEISG LWNELDSLKD 1020
AVEHQRKKNN DLREKNWEAM EALASTEKML QDKVNKTSKE RQQQVEAVEL EAKEVLKKLF 1080
PKVSVPSNLS YGEWLHGFEK KAKECMAGTS GSEEVKVLEH KLKEADEMHT LLQLECEKYK 1140
SVLAETEGIL QKLQRSVEQE ENKWKVKVDE SHKTIKQMQS SFTSSEQELE RLRSENKDIE 1200
NLRREREHLE MELEKAEMER STYVTEVREL KDLLTELQKK LDDSYSEAVR QNEELNLLKA 1260
QLNETLTKLR TEQNERQKVA GDLHKAQQSL ELIQSKIVKA AGDTTVIENS DVSPETESSE 1320
KETMSVSLNQ TVTQLQQLLQ AVNQQLTKEK EHYQVLE 1357 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:ProtInc.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0007018; P:microtubule-based movement; NAS:ProtInc. 
Interpro
 IPR024854; Kinectin. 
Pfam
  
SMART
  
PROSITE
  
PRINTS