CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019030
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exocyst complex component 4 
Protein Synonyms/Alias
 Exocyst complex component Sec8 
Gene Name
 EXOC4 
Gene Synonyms/Alias
 KIAA1699; SEC8; SEC8L1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9AAEAAGGKYRSTVSKacetylation[1, 2]
9AAEAAGGKYRSTVSKubiquitination[3, 4]
18RSTVSKSKDPSGLLIubiquitination[2, 4]
101KENLLSCKMLLHCKRubiquitination[4]
113CKRDELRKLWIEGIEubiquitination[4]
140NIKQVPQKLEQCMASubiquitination[4]
217QRNKEKGKISSLVKDubiquitination[4]
223GKISSLVKDASVPLIubiquitination[4, 5, 6]
240TNLPTPRKFLDTSHYubiquitination[4, 5, 6]
432FAAFFAKKKPQRPKNubiquitination[4]
486LIEGGGTKFVCKPGAubiquitination[4]
490GGTKFVCKPGARNITubiquitination[4]
662WMNMAQPKQLRPKREubiquitination[4]
953NTRLQRLKEIICEQAubiquitination[4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 9 9 N6-acetyllysine.
 MOD_RES 237 237 Phosphothreonine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Direct protein sequencing; Exocytosis; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 974 AA 
Protein Sequence
MAAEAAGGKY RSTVSKSKDP SGLLISVIRT LSTSDDVEDR ENEKGRLEEA YEKCDRDLDE 60
LIVQHYTELT TAIRTYQSIT ERITNSRNKI KQVKENLLSC KMLLHCKRDE LRKLWIEGIE 120
HKHVLNLLDE IENIKQVPQK LEQCMASKHY LSATDMLVSA VESLEGPLLQ VEGLSDLRLE 180
LHSKKMNLHL VLIDELHRHL YIKSTSRVVQ RNKEKGKISS LVKDASVPLI DVTNLPTPRK 240
FLDTSHYSTA GSSSVREINL QDIKEDLELD PEENSTLFMG ILIKGLAKLK KIPETVKAII 300
ERLEQELKQI VKRSTTQVAD SGYQRGENVT VENQPRLLLE LLELLFDKFN AVAAAHSVVL 360
GYLQDTVVTP LTQQEDIKLY DMADVWVKIQ DVLQMLLTEY LDMKNTRTAS EPSAQLSYAS 420
TGREFAAFFA KKKPQRPKNS LFKFESSSHA ISMSAYLREQ RRELYSRSGE LQGGPDDNLI 480
EGGGTKFVCK PGARNITVIF HPLLRFIQEI EHALGLGPAK QCPLREFLTV YIKNIFLNQV 540
LAEINKEIEG VTKTSDPLKI LANADTMKVL GVQRPLLQST IIVEKTVQDL LNLMHDLSAY 600
SDQFLNMVCV KLQEYKDTCT AAYRGIVQSE EKLVISASWA KDDDISRLLK SLPNWMNMAQ 660
PKQLRPKREE EEDFIRAAFG KESEVLIGNL GDKLIPPQDI LRDVSDLKAL ANMHESLEWL 720
ASRTKSAFSN LSTSQMLSPA QDSHTNTDLP PVSEQIMQTL SELAKSFQDM ADRCLLVLHL 780
EVRVHCFHYL IPLAKEGNYA IVANVESMDY DPLVVKLNKD ISAIEEAMSA SLQQHKFQYI 840
FEGLGHLISC ILINGAQYFR RISESGIKKM CRNIFVLQQN LTNITMSREA DLDFARQYYE 900
MLYNTADELL NLVVDQGVKY TELEYIHALT LLHRSQTGVG ELTTQNTRLQ RLKEIICEQA 960
AIKQATKDKK ITTV 974 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:LIFEdb.
 GO:0000145; C:exocyst; IEA:InterPro.
 GO:0032584; C:growth cone membrane; IEA:Compara.
 GO:0035748; C:myelin sheath abaxonal region; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006904; P:vesicle docking involved in exocytosis; IEA:InterPro. 
Interpro
 IPR007191; Sec8_exocyst. 
Pfam
 PF04048; Sec8_exocyst 
SMART
  
PROSITE
  
PRINTS