CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024432
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Adenosylhomocysteinase 
Protein Synonyms/Alias
 S-adenosyl-L-homocysteine hydrolase; AdoHcyase 
Gene Name
 ahcY 
Gene Synonyms/Alias
 MSMEG_1843; MSMEI_1801 
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
464GTLTKLSKDQAEYIGpupylation[1]
Reference
 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657] 
Functional Description
 May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine (By similarity). 
Sequence Annotation
 NP_BIND 210 212 NAD (By similarity).
 NP_BIND 273 278 NAD (By similarity).
 NP_BIND 352 354 NAD (By similarity).
 BINDING 64 64 Substrate (By similarity).
 BINDING 147 147 Substrate (By similarity).
 BINDING 209 209 Substrate (By similarity).
 BINDING 239 239 Substrate (By similarity).
 BINDING 243 243 Substrate (By similarity).
 BINDING 244 244 NAD (By similarity).
 BINDING 296 296 NAD (By similarity).
 BINDING 331 331 NAD (By similarity).
 BINDING 399 399 NAD (By similarity).
 CROSSLNK 464 464 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 Complete proteome; Cytoplasm; Hydrolase; Isopeptide bond; NAD; One-carbon metabolism; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 485 AA 
Protein Sequence
MTELKADVRN GIDYKVADLS LADFGRKEIR LAEHEMPGLM ALRREYHDVQ PLKGARISGS 60
LHMTVQTAVL IETLVSLGAE VRWASCNIFS TQDHAAAAVV VGPNGTPEEP KGVSVFAWKG 120
ETLEEYWWAA EQMLTWDGEP ANMILDDGGD ATMMVLRGAQ YEKAGVVPPA EDDDPAEWKV 180
FLGVLRERFE QDKTKWTKIA ESVKGVTEET TTGVLRLYQF AAAGELAFPA INVNDSVTKS 240
KFDNKYGTRH SLIDGINRGT DVLIGGKKVL ICGYGDVGKG CAESLAGQGA RVSVTEIDPI 300
NALQALMDGF DVRTVEEAIG EADIVITATG NKDIITLDHM KAMKNQAILG NIGHFDNEID 360
MAALERSGAK KINIKPQVDQ WIFDDGKSII VLSEGRLLNL GNATGHPSFV MSNSFSNQVI 420
AQIELWTKND EYDNEVYRLA KHLDEKVARI HVEALGGTLT KLSKDQAEYI GVDVEGPYKP 480
EHYRY 485 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004013; F:adenosylhomocysteinase activity; IEA:HAMAP.
 GO:0006730; P:one-carbon metabolic process; IEA:HAMAP. 
Interpro
 IPR000043; Adenosylhomocysteinase.
 IPR015878; Ado_hCys_hydrolase_NAD-bd.
 IPR016040; NAD(P)-bd_dom.
 IPR020082; S-Ado-L-homoCys_hydrolase_CS. 
Pfam
 PF05221; AdoHcyase
 PF00670; AdoHcyase_NAD 
SMART
 SM00996; AdoHcyase
 SM00997; AdoHcyase_NAD 
PROSITE
 PS00738; ADOHCYASE_1
 PS00739; ADOHCYASE_2 
PRINTS