CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008177
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleoporin NUP145 
Protein Synonyms/Alias
 Nuclear pore protein NUP145; Nucleoporin NUP145N; N-NUP145; Nucleoporin NUP145C; C-NUP145 
Gene Name
 NUP145 
Gene Synonyms/Alias
 RAT10; YGL092W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
664TFGTLSGKDDSIVEEubiquitination[1]
1198PVQNNELKTLREILNubiquitination[1]
1299LPLGQPEKAYLRGEFubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP145 is autocatalytically cleaved in vivo in 2 polypeptides which assume different functions in the NPC. NUP145N as one of the FG repeat nucleoporins participates in karyopherin interactions and contains part of the autocatalytic cleavage activity. NUP145C as part of the NUP84 complex is involved in nuclear poly(A)+ RNA and tRNA export. It is also required for normal NPC distribution (probably through interactions with MLP1 and MLP2) and NPC assembly, as well as for normal nuclear envelope organization. 
Sequence Annotation
 REPEAT 12 13 FG 1.
 REPEAT 39 42 GLFG 1.
 REPEAT 79 80 FG 2.
 REPEAT 89 92 GLFG 2.
 REPEAT 106 107 FG 3.
 REPEAT 136 139 GLFG 3.
 REPEAT 154 157 GLFG 4.
 REPEAT 168 171 GLFG 5.
 REPEAT 181 184 GLFG 6.
 REPEAT 193 196 GLFG 7; approximate.
 REPEAT 206 209 GLFG 8.
 DOMAIN 458 605 Peptidase S59.
 REGION 398 523 Required for autocatalytic cleavage.
 REGION 460 604 Nucleoporin RNA-binding motif (NRM).
 MOTIF 369 385 Bipartite nuclear localization signal
 MOD_RES 273 273 Phosphoserine.
 MOD_RES 403 403 Phosphoserine.
 MOD_RES 404 404 Phosphoserine.
 MOD_RES 414 414 Phosphoserine.
 MOD_RES 667 667 Phosphoserine.
 MOD_RES 679 679 Phosphoserine.
 MOD_RES 689 689 Phosphoserine.
 MOD_RES 751 751 Phosphothreonine.  
Keyword
 3D-structure; Autocatalytic cleavage; Complete proteome; Hydrolase; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; RNA-binding; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1317 AA 
Protein Sequence
MFNKSVNSGF TFGNQNTSTP TSTPAQPSSS LQFPQKSTGL FGNVNVNANT STPSPSGGLF 60
NANSNANSIS QQPANNSLFG NKPAQPSGGL FGATNNTTSK SAGSLFGNNN ATANSTGSTG 120
LFSGSNNIAS STQNGGLFGN SNNNNITSTT QNGGLFGKPT TTPAGAGGLF GNSSSTNSTT 180
GLFGSNNTQS STGIFGQKPG ASTTGGLFGN NGASFPRSGE TTGTMSTNPY GINISNVPMA 240
VADMPRSITS SLSDVNGKSD AEPKPIENRR TYSFSSSVSG NAPLPLASQS SLVSRLSTRL 300
KATQKSTSPN EIFSPSYSKP WLNGAGSAPL VDDFFSSKMT SLAPNENSIF PQNGFNFLSS 360
QRADLTELRK LKIDSNRSAA KKLKLLSGTP AITKKHMQDE QDSSENEPIA NADSVTNIDR 420
KENRDNNLDN TYLNGKEQSN NLNKQDGENT LQHEKSSSFG YWCSPSPEQL ERLSLKQLAA 480
VSNFVIGRRG YGCITFQHDV DLTAFTKSFR EELFGKIVIF RSSKTVEVYP DEATKPMIGH 540
GLNVPAIITL ENVYPVDKKT KKPMKDTTKF AEFQVFDRKL RSMREMNYIS YNPFGGTWTF 600
KVNHFSIWGL VNEEDAEIDE DDLSKQEDGG EQPLRKVRTL AQSKPSDKEV ILKTDGTFGT 660
LSGKDDSIVE EKAYEPDLSD ADFEGIEASP KLDVSKDWVE QLILAGSSLR SVFATSKEFD 720
GPCQNEIDLL FSECNDEIDN AKLIMKERRF TASYTFAKFS TGSMLLTKDI VGKSGVSIKR 780
LPTELQRKFL FDDVYLDKEI EKVTIEARKS NPYPQISESS LLFKDALDYM EKTSSDYNLW 840
KLSSILFDPV SYPYKTDNDQ VKMALLKKER HCRLTSWIVS QIGPEIEEKI RNSSNEIEQI 900
FLYLLLNDVV RASKLAIESK NGHLSVLISY LGSNDPRIRD LAELQLQKWS TGGCSIDKNI 960
SKIYKLLSGS PFEGLFSLKE LESEFSWLCL LNLTLCYGQI DEYSLESLVQ SHLDKFSLPY 1020
DDPIGVIFQL YAANENTEKL YKEVRQRTNA LDVQFCWYLI QTLRFNGTRV FSKETSDEAT 1080
FAFAAQLEFA QLHGHSLFVS CFLNDDKAAE DTIKRLVMRE ITLLRASTND HILNRLKIPS 1140
QLIFNAQALK DRYEGNYLSE VQNLLLGSSY DLAEMAIVTS LGPRLLLSNN PVQNNELKTL 1200
REILNEFPDS ERDKWSVSIN VFEVYLKLVL DNVETQETID SLISGMKIFY DQYKHCREVA 1260
ACCNVMSQEI VSKILEKNNP SIGDSKAKLL ELPLGQPEKA YLRGEFAQDL MKCTYKI 1317 
Gene Ontology
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0044613; C:nuclear pore central transport channel; IDA:SGD.
 GO:0031080; C:nuclear pore outer ring; IDA:SGD.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0005487; F:nucleocytoplasmic transporter activity; IGI:SGD.
 GO:0003723; F:RNA binding; IDA:SGD.
 GO:0017056; F:structural constituent of nuclear pore; IMP:SGD.
 GO:0006302; P:double-strand break repair; IMP:SGD.
 GO:0035392; P:maintenance of chromatin silencing at telomere; IMP:SGD.
 GO:0006607; P:NLS-bearing substrate import into nucleus; IGI:SGD.
 GO:0031081; P:nuclear pore distribution; IMP:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:SGD.
 GO:0000973; P:posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery; IMP:SGD.
 GO:0036228; P:protein targeting to nuclear inner membrane; IGI:SGD.
 GO:0034398; P:telomere tethering at nuclear periphery; IMP:SGD.
 GO:0006409; P:tRNA export from nucleus; IMP:SGD. 
Interpro
 IPR025574; Nucleoporin_FG_rpt.
 IPR021967; Nup96.
 IPR007230; Peptidase_S59. 
Pfam
 PF04096; Nucleoporin2
 PF13634; Nucleoporin_FG
 PF12110; Nup96 
SMART
  
PROSITE
 PS51434; NUP_C 
PRINTS