CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001282
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Signal transducing adapter molecule 2 
Protein Synonyms/Alias
 STAM-2; Hrs-binding protein 
Gene Name
 STAM2 
Gene Synonyms/Alias
 HBP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
57IMKRVNHKVPHVALQubiquitination[1]
122EWSEEFQKDPQFSLIubiquitination[2, 3, 4, 5]
134SLISATIKSMKEEGIubiquitination[2, 5, 6, 7]
156QTVSAAAKNGTSSNKubiquitination[2, 5]
165GTSSNKNKEDEDIAKubiquitination[2]
182ELSLQEQKQQHTETKubiquitination[1, 2, 3, 5]
189KQQHTETKSLYPSSEubiquitination[1, 2, 3, 5, 6, 7]
202SEIQLNNKVARKVRAubiquitination[1, 2, 5, 6, 7, 8]
283DDDVEEIKKSEPEPVubiquitination[2, 5]
353ELSELNVKVLEALELubiquitination[1]
363EALELYNKLVNEAPVubiquitination[2, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity). 
Sequence Annotation
 DOMAIN 16 144 VHS.
 REPEAT 165 184 UIM.
 DOMAIN 202 261 SH3.
 DOMAIN 360 377 ITAM.
 REGION 219 220 Interaction with USP8 (By similarity).
 REGION 334 368 Interaction with HGS (By similarity).
 MOTIF 54 67 PxVxL motif.
 MOD_RES 374 374 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Endosome; Membrane; Phosphoprotein; Protein transport; Reference proteome; SH3 domain; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 525 AA 
Protein Sequence
MPLFTANPFE QDVEKATNEY NTTEDWSLIM DICDKVGSTP NGAKDCLKAI MKRVNHKVPH 60
VALQALTLLG ACVANCGKIF HLEVCSRDFA TEVRAVIKNK AHPKVCEKLK SLMVEWSEEF 120
QKDPQFSLIS ATIKSMKEEG ITFPPAGSQT VSAAAKNGTS SNKNKEDEDI AKAIELSLQE 180
QKQQHTETKS LYPSSEIQLN NKVARKVRAL YDFEAVEDNE LTFKHGEIII VLDDSDANWW 240
KGENHRGIGL FPSNFVTTNL NIETEAAAVD KLNVIDDDVE EIKKSEPEPV YIDEDKMDRA 300
LQVLQSIDPT DSKPDSQDLL DLEDICQQMG PMIDEKLEEI DRKHSELSEL NVKVLEALEL 360
YNKLVNEAPV YSVYSKLHPP AHYPPASSGV PMQTYPVQSH GGNYMGQSIH QVTVAQSYSL 420
GPDQIGPLRS LPPNVNSSVT AQPAQTSYLS TGQDTVSNPT YMNQNSNLQS ATGTTAYTQQ 480
MGMSVDMSSY QNTTSNLPQL AGFPVTVPAH PVAQQHTNYH QQPLL 525 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0016197; P:endosomal transport; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR008942; ENTH_VHS.
 IPR000108; p67phox.
 IPR001452; SH3_domain.
 IPR003903; Ubiquitin-int_motif.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF00018; SH3_1
 PF02809; UIM
 PF00790; VHS 
SMART
 SM00326; SH3
 SM00726; UIM
 SM00288; VHS 
PROSITE
 PS51055; ITAM_1
 PS50002; SH3
 PS50330; UIM
 PS50179; VHS 
PRINTS
 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.