CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000727
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mothers against decapentaplegic homolog 6 
Protein Synonyms/Alias
 MAD homolog 6; Mothers against DPP homolog 6; SMAD family member 6; SMAD 6; Smad6; hSMAD6 
Gene Name
 SMAD6 
Gene Synonyms/Alias
 MADH6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
470SVRISFAKGWGPCYSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions. 
Sequence Annotation
 DOMAIN 148 275 MH1.
 DOMAIN 331 496 MH2.
 METAL 205 205 Zinc (By similarity).
 METAL 247 247 Zinc (By similarity).
 METAL 260 260 Zinc (By similarity).
 METAL 265 265 Zinc (By similarity).
 MOD_RES 435 435 Phosphoserine; by PRKX; in vitro.  
Keyword
 Alternative splicing; Complete proteome; Disease mutation; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 496 AA 
Protein Sequence
MFRSKRSGLV RRLWRSRVVP DREEGGSGGG GGGDEDGSLG SRAEPAPRAR EGGGCGRSEV 60
RPVAPRRPRD AVGQRGAQGA GRRRRAGGPP RPMSEPGAGA GSSLLDVAEP GGPGWLPESD 120
CETVTCCLFS ERDAAGAPRD ASDPLAGAAL EPAGGGRSRE ARSRLLLLEQ ELKTVTYSLL 180
KRLKERSLDT LLEAVESRGG VPGGCVLVPR ADLRLGGQPA PPQLLLGRLF RWPDLQHAVE 240
LKPLCGCHSF AAAADGPTVC CNPYHFSRLC GPESPPPPYS RLSPRDEYKP LDLSDSTLSY 300
TETEATNSLI TAPGEFSDAS MSPDATKPSH WCSVAYWEHR TRVGRLYAVY DQAVSIFYDL 360
PQGSGFCLGQ LNLEQRSESV RRTRSKIGFG ILLSKEPDGV WAYNRGEHPI FVNSPTLDAP 420
GGRALVVRKV PPGYSIKVFD FERSGLQHAP EPDAADGPYD PNSVRISFAK GWGPCYSRQF 480
ITSCPCWLEI LLNNPR 496 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005667; C:transcription factor complex; IEA:InterPro.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0030617; F:transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; IDA:BHF-UCL.
 GO:0070698; F:type I activin receptor binding; IDA:BHF-UCL.
 GO:0034713; F:type I transforming growth factor beta receptor binding; IDA:BHF-UCL.
 GO:0030509; P:BMP signaling pathway; TAS:Reactome.
 GO:0031589; P:cell-substrate adhesion; IMP:UniProtKB.
 GO:0006955; P:immune response; IMP:BHF-UCL.
 GO:0043066; P:negative regulation of apoptotic process; IMP:BHF-UCL.
 GO:0030514; P:negative regulation of BMP signaling pathway; IDA:BHF-UCL.
 GO:0008285; P:negative regulation of cell proliferation; IMP:BHF-UCL.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IMP:BHF-UCL.
 GO:0060394; P:negative regulation of pathway-restricted SMAD protein phosphorylation; IDA:BHF-UCL.
 GO:0010991; P:negative regulation of SMAD protein complex assembly; IDA:BHF-UCL.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IMP:BHF-UCL.
 GO:0043627; P:response to estrogen stimulus; IEA:Compara.
 GO:0034616; P:response to laminar fluid shear stress; IEP:BHF-UCL.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; IEA:InterPro.
 GO:0001657; P:ureteric bud development; IEA:Compara.
 GO:0007352; P:zygotic specification of dorsal/ventral axis; IMP:BHF-UCL. 
Interpro
 IPR013790; Dwarfin.
 IPR003619; MAD_homology1_Dwarfin-type.
 IPR013019; MAD_homology_MH1.
 IPR017855; SMAD_dom-like.
 IPR001132; SMAD_dom_Dwarfin-type.
 IPR008984; SMAD_FHA_domain. 
Pfam
 PF03165; MH1
 PF03166; MH2 
SMART
 SM00523; DWA
 SM00524; DWB 
PROSITE
 PS51075; MH1
 PS51076; MH2 
PRINTS