CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015263
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteasome-associated protein ECM29 homolog 
Protein Synonyms/Alias
 Ecm29 
Gene Name
 Ecm29 
Gene Synonyms/Alias
 Kiaa0368 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
40FLPPVLLKLSSTQEGubiquitination[1]
150KYPAESSKSASPFNLubiquitination[1]
280TAADLELKSKQSLIDubiquitination[1]
308YLGDIPLKTKEGAVLubiquitination[1]
310GDIPLKTKEGAVLKPubiquitination[1]
387TCPEIKIKPLGPMLLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis (By similarity). 
Sequence Annotation
 REPEAT 28 65 HEAT 1.
 REPEAT 107 144 HEAT 2.
 REPEAT 162 199 HEAT 3.
 REPEAT 327 363 HEAT 4.
 REPEAT 388 427 HEAT 5.
 REPEAT 430 467 HEAT 6.
 REPEAT 470 508 HEAT 7.
 REPEAT 722 760 HEAT 8.
 REPEAT 773 810 HEAT 9.
 REPEAT 830 869 HEAT 10.
 REPEAT 871 908 HEAT 11.
 REPEAT 932 970 HEAT 12.
 REPEAT 976 1013 HEAT 13.
 REPEAT 1014 1051 HEAT 14.
 REPEAT 1113 1150 HEAT 15.
 REPEAT 1153 1190 HEAT 16.
 REPEAT 1195 1232 HEAT 17.
 REPEAT 1244 1282 HEAT 18.
 REPEAT 1286 1324 HEAT 19.
 REPEAT 1349 1387 HEAT 20.
 REPEAT 1391 1428 HEAT 21.
 REPEAT 1518 1555 HEAT 22.
 REPEAT 1559 1596 HEAT 23.
 REPEAT 1659 1696 HEAT 24.
 REPEAT 1780 1823 HEAT 25.
 MOD_RES 825 825 Phosphoserine (By similarity).
 MOD_RES 831 831 Phosphothreonine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endoplasmic reticulum; Endosome; Nucleus; Phosphoprotein; Proteasome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1840 AA 
Protein Sequence
METGSDSDQL ERVFLRLGHA ETDEQLQNII SKFLPPVLLK LSSTQEGVRK KVMELLVHLN 60
KRIKSRPKIQ LPVETLLVQY QDPAAVSFVT NFTIIYVKMG YPRLPVEKQC ELAPTLLTAM 120
EGKPQPQQDS LMHLLIPTLF HMKYPAESSK SASPFNLAEK PKTVQLLLDF MLDVLLMPYG 180
YVLNESQSRQ NSSSSSQGSS SNSGGGSGIP QPPPGMSFYA AKRVIGDNPW TPEQLEQCKL 240
GIVKFIEAEQ VPELEAVLHL VIASSDTRHS VATAADLELK SKQSLIDWNN PAIINKMYKV 300
YLGDIPLKTK EGAVLKPELK RDPVSTRVKL KIVPHLLRSR QAAETFPANI QVVYDGLFGT 360
NTNSKLRTLS LQFVHHICLT CPEIKIKPLG PMLLNGLTKL INEYKEDPKL LSMAYSAVGK 420
LSSRMPHLFT KDIALVQQLF EALCKEEPET RLAIQEALSM MVGAYSTLEG AQRTLMEALV 480
ASYLIKPEVQ VRQVAVKFAS TVFPSDHIPS RYLLLLAAGD PREEVHGEAQ RVLRCLPGRN 540
KKESASKQMP SFPEMVYYIQ EKASHRMKTP VKYMTGTTVL PFNPAAFGEI VLYLRMCLAH 600
SAGVVPTSQS LADMQDHAPA IGRYIRALMS SSQATASSSN KSGETNPVQI YTGLLQQLLA 660
GVGGLPVMYC LLEAVSVYPE KLATKFVDKT EWIKSLMSSS KEEMRELAAL FYSVVVSTVS 720
GIELKSMIEQ LIKATKDNHS PEVQHGSLLA LGFTVGRYLA KKRVRMAEQH DLETDADLLP 780
EQEEIIRSAT ETIGSFLDST SPLLAIAACT ALGEIGRNGP LPIPSEGSGF TKLHLVESLL 840
NRIPSSKETN KMKERAIQTL GYFPVGDGVF PHQKLLLQGL MDSVEAKQIE LQFTIGEAIT 900
SAAIGTNSVA ARDAWLVTEE EYIPPAGAKV NDVVPWVLDV ILNKHIISPN PHVRQAACIW 960
LLSLVRKLST HREVKSHLKE IQSAFVSVLS ENDELSQDVA SKGLGLVYEL GNEQDQQELV 1020
STLVETLMTG KRVKHEVSGE TVVFQGGGLG KTPDGQGLST YKELCSLASD LSQPDLVYKF 1080
MNLANHHAMW NSRKGAAFGF NVIATRAGEQ LAPFLPQLVP RLYRYQFDPN LGIRQAMTSI 1140
WNALVTDKSM VDKYLKEILQ DLIKNLTSNM WRVRESSCLA LNDLLRGRPL DDVIDKLPEM 1200
WETLFRVQDD IKESVRKAAE LALKTLSKVC VKMCDPAKGA AGQRTIAVLL PCLLDKGMMS 1260
PVTEVRALSI NTLVKISKSA GAMLKPHAPK LIPALLESLS VLEPQVLNYL SLRATEQEKD 1320
VMDSARLSAA KSSPMMETIN MCLQYLDVSV LGELVPRLCE LIRSGVGLGT KGGCASVIVS 1380
LTTQCPQDLT PYSGKLMSAL LSGLTDRNSV IQKSCAFAMG HLVRTSRDSS TEKLLQKLNG 1440
WYMEKDEPVY KTSCALTIHA IGRYSPDVLK NHAKEVLPLA FLGMHEIADE EKSEKEECNM 1500
WTEVWQENVP GSFGGIRLYL QELITITQKA LQSQSWKMKA QGAIAMASIS KQTSSLVPPY 1560
LGMILSALMQ GLAGRTWAGK EELLKAIACV VTACSTELEK SVPNQPTTNE ILQAVLKECC 1620
KENLKYKIVA ISCAADVLKA TKEDRFQEFS DIVIPLIKKN SLESMGVRTT KAEDENEKER 1680
ELQLESLLGA FESLGKAWPR NPDTQRCYRQ ELCKLMCERL RLSTWKVQLG VLQSMNAFFQ 1740
GLMLLEEEHA DPEALAEILL ETCKSITYSL ENKTYSSVRT EALSVVELLL KKLEEAKQWE 1800
SLTAECRGLL IESLATMETD NRPELQEKAS VLKKTLESLE 1840 
Gene Ontology
 GO:0030139; C:endocytic vesicle; ISS:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005771; C:multivesicular body; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR026827; ECM29/GCN1.
 IPR026826; ECM29_metazoa.
 IPR024372; Proteasome_stabil_ECM29. 
Pfam
 PF13001; Ecm29 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS