CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018563
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neural Wiskott-Aldrich syndrome protein 
Protein Synonyms/Alias
 N-WASP 
Gene Name
 Wasl 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
183VNNISHTKEKKKGKAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Regulates actin polymerization by stimulating the actin- nucleating activity of the Arp2/3 complex. Binds to TNK2 (By similarity). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. 
Sequence Annotation
 DOMAIN 31 138 WH1.
 DOMAIN 200 213 CRIB.
 DOMAIN 401 418 WH2 1.
 DOMAIN 429 446 WH2 2.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 239 239 Phosphoserine; by TNK2 (By similarity).
 MOD_RES 253 253 Phosphotyrosine; by FAK1 and TNK2.
 MOD_RES 480 480 Phosphoserine (By similarity).
 MOD_RES 481 481 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 501 AA 
Protein Sequence
MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR NCMWAKKCSG 60
VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP RGYFHTFAGD TCQVALNFAN 120
EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN GPNLPMATVD IKNPEITTNR FYGSQVNNIS 180
HTKEKKKGKA KKKRLTKADI GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE 240
AQLKDRETSK VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP 300
ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS APSGPPPPPP 360
PSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPAPSG NKAALLDQIR EGAQLKKVEQ 420
NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ ESTPPTPAPT SGIVGALMEV MQKRSKAIHS 480
SDEDEDDDDE EDFEDDDEWE D 501 
Gene Ontology
 GO:0030478; C:actin cap; IDA:MGI.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; ISS:BHF-UCL.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030027; C:lamellipodium; IDA:MGI.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005083; F:small GTPase regulator activity; IEA:InterPro.
 GO:0045010; P:actin nucleation; TAS:MGI.
 GO:0008154; P:actin polymerization or depolymerization; IEA:InterPro.
 GO:0034629; P:cellular protein complex localization; IMP:MGI.
 GO:0006900; P:membrane budding; ISS:BHF-UCL.
 GO:2000370; P:positive regulation of clathrin-mediated endocytosis; ISS:BHF-UCL.
 GO:0051491; P:positive regulation of filopodium assembly; ISS:BHF-UCL.
 GO:0006461; P:protein complex assembly; IEA:InterPro.
 GO:0032880; P:regulation of protein localization; IMP:MGI.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0009617; P:response to bacterium; IMP:MGI.
 GO:0051653; P:spindle localization; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0030050; P:vesicle transport along actin filament; ISS:BHF-UCL. 
Interpro
 IPR000697; EVH1.
 IPR000095; PAK_box_Rho-bd.
 IPR011993; PH_like_dom.
 IPR011026; WASP_C.
 IPR003124; WH2_dom. 
Pfam
 PF00786; PBD
 PF00568; WH1
 PF02205; WH2 
SMART
 SM00285; PBD
 SM00461; WH1
 SM00246; WH2 
PROSITE
 PS50108; CRIB
 PS50229; WH1
 PS51082; WH2 
PRINTS