UniProt Accession

 G3PC2_ARATH; Q9FX54; B9DGZ4 

Genbank Protein ID

 AC011810; CP002684; AF410271; AY049259; AY090275; AK317337 

Genbank Nucleotide ID

 AAG09543.1; AEE29016.1; AAK95257.1; AAK83601.1; AAL90936.1; BAH20011.1 

Protein Name

 Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic 

Protein Synonyms/Alias

 NAD-dependent glyceraldehydephosphate dehydrogenase C subunit 2 

Gene Name

 GAPC2 

Gene Synonyms/Alias

 GAPDH; At1g13440; T6J4.17 

Created Date

 July 27, 2013 

Organism

 Arabidopsis thaliana (Mouse-ear cress) 

NCBI Taxa ID

 3702 

Lysine Modification

Position	Peptide	Type	References
130	VVISAPSKDAPMFVV	acetylation	[1]
219	PSSTGAAKAVGKVLP	acetylation	[1]
223	GAAKAVGKVLPSLNG	acetylation	[1]
255	DLTVRLEKAATYDEI	acetylation	[1]

Reference

 [1] Proteins of diverse function and subcellular location are lysine acetylated in Arabidopsis.
 Finkemeier I, Laxa M, Miguet L, Howden AJ, Sweetlove LJ.
 Plant Physiol. 2011 Apr;155(4):1779-90. [PMID: 21311031] 

Functional Description

 Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism (By similarity). Binds DNA in vitro. 

Sequence Annotation

 NP_BIND 15 16 NAD (By similarity).
 REGION 155 157 Glyceraldehyde 3-phosphate binding (By
 REGION 215 216 Glyceraldehyde 3-phosphate binding (By
 ACT_SITE 156 156 Nucleophile (By similarity).
 BINDING 37 37 NAD (By similarity).
 BINDING 84 84 NAD; via carbonyl oxygen (By similarity).
 BINDING 186 186 Glyceraldehyde 3-phosphate (By
 BINDING 238 238 Glyceraldehyde 3-phosphate (By
 BINDING 320 320 NAD (By similarity).
 MOD_RES 156 156 S-glutathionyl cysteine; transient.
 MOD_RES 156 156 S-nitrosocysteine; transient.
 MOD_RES 160 160 S-nitrosocysteine; transient.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; DNA-binding; Glutathionylation; Glycolysis; NAD; Nucleus; Oxidoreductase; Reference proteome; S-nitrosylation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 338 AA 

Protein Sequence

Gene Ontology

 GO:0005618; C:cell wall; IDA:TAIR.
 GO:0009507; C:chloroplast; IDA:TAIR.
 GO:0005829; C:cytosol; IDA:TAIR.
 GO:0005739; C:mitochondrion; IDA:TAIR.
 GO:0005730; C:nucleolus; IDA:TAIR.
 GO:0005886; C:plasma membrane; IDA:TAIR.
 GO:0009506; C:plasmodesma; IDA:TAIR.
 GO:0005507; F:copper ion binding; IDA:TAIR.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:UniProtKB.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IDA:TAIR.
 GO:0042742; P:defense response to bacterium; IEP:TAIR.
 GO:0006094; P:gluconeogenesis; TAS:TAIR.
 GO:0006096; P:glycolysis; TAS:TAIR.
 GO:0046686; P:response to cadmium ion; IEP:TAIR.
 GO:0006979; P:response to oxidative stress; IDA:TAIR. 

Interpro

 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 

SMART

 SM00846; Gp_dh_N 

PROSITE

 PS00071; GAPDH 

PRINTS

 PR00078; G3PDHDRGNASE.