| Tag | Content |
|---|
| CPLM ID | CPLM-007058 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Mediator of RNA polymerase II transcription subunit 13 |
Protein Synonyms/Alias | Mediator complex subunit 13; Protein SCA1; Suppressor of RNA polymerase B SSN2 |
Gene Name | SSN2 |
Gene Synonyms/Alias | MED13; NUT8; RYE3; SCA1; SRB9; UME2; YDR443C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 632 | NKYKSGGKFSFSPLQ | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II- dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. |
Sequence Annotation | MOD_RES 370 370 Phosphoserine.
MOD_RES 375 375 Phosphoserine.
MOD_RES 425 425 Phosphoserine.
MOD_RES 601 601 Phosphothreonine.
MOD_RES 608 608 Phosphoserine; by PKA.
MOD_RES 636 636 Phosphoserine.
MOD_RES 748 748 Phosphoserine. |
Keyword | Activator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1420 AA |
Protein Sequence | MSSDASTYRL EDVLSSFYRV EKIKKINYHQ YISKAQNDQW SIQMEFMLRK QDPKTLVALL 60
SRDLWCFSIN DDPVPTPPAI EHKPVSPDKI GTFTADYSKP NLPPHYALFL KALRRKIYIN 120
LALGSHNKLI QFGNACISLS GVPNYLVQLE PHLFVNGDLT VSLCAKNMGL VPMKEENLEE 180
SFLSKHALYL APSGIRMHLA PASKQGYLIT PPKHTELLLT TLSVSHGINL QNKKNLKWVA 240
VVPDLGHLNG HTPTIASYLT PLLEAKKLVW PLHLIFAQPV ADIENSTSGD PSEFHCLQDA 300
LDAIDDFIQL KQTAAYRTPG SSGVLSSNIA GTNPLSSDGA YTEQFQHYKN NSISSQPASY 360
HSVQETNKIS PKDFSPNFTG IDKLMLSPSD QFAPAFLNTP NNNINENELF NDRKQTTVSN 420
DLENSPLKTE LEANGRSLEK VNNSVSKTGS VDTLHNKEGT LEQREQNENL PSDKSDSMVD 480
KELFGEDEDE DLFGDSNKSN STNESNKSIS DEITEDMFEM SDEEENNNNK SINKNNKEMH 540
TDLGKDIPFF PSSEKPNIRT MSGTTKRLNG KRKYLDIPID EMTLPTSPLY MDPGAPLPVE 600
TPRDRRKSVF APLNFNPIIE NNVDNKYKSG GKFSFSPLQK EEALNFDISM ADLSSSEEEE 660
DEEENGSSDE DLKSLNVRDD MKPSDNISTN TNIHEPQYIN YSSIPSLQDS IIKQENFNSV 720
NDANITSNKE GFNSIWKIPQ NDIPQTESPL KTVDSSIQPI ESNIKMTLED NNVTSNPSEF 780
TPNMVNSEIS NLPKDKSGIP EFTPADPNLS FESSSSLPFL LRHMPLASIP DIFITPTPVV 840
TISEKEQDIL DLIAEQVVTD YNILGNLGIP KIAYRGVKDC QEGLITTTML QLFSTFDRLN 900
GNDTISKFYN MKQPYVFVKK HHELIKVKHD SQPFIKFLNF RPPNGIKNFK SLLLSSSFKE 960
DCLSFAPTLS QTYINQELGF CELLKLTNED PPGLMYLKAF DKNKLLLLAA QIVSYCSNNK 1020
NSIKNVPPIL IILPLDNATL TELVDKANIF QVIKNEVCAK MPNIELYLKV IPMDFIRNVL 1080
VTVDQYVNVA ISIYNMLPPK SVKFTHIAHT LPEKVNFRTM QQQQMQQQQQ QQQQQQNNST 1140
GSSSIIYYDS YIHLAYSRSV DKEWVFAALS DSYGQGSMTK TWYVGNSRGK FDDACNQIWN 1200
IALNLASKKF GKICLILTRL NGILPDDELM NWRRLSGRNI HLAVVCVDDN SKISFIDEDK 1260
LYPSFKPIYK DTRFGGRMDM TRLYDYEIRD IDQDIHGIVF QHPFPLAHSQ HRCAIRSGAL 1320
IKFKKCDGDT VWDKFAVNLL NCPHSDSTQL LETILEEFRN LAALNVWYGL SDGEDGHIPW 1380
HILAVKKMMN TLVHTRVKIA NTSAATVHTA TSSSIILSDK 1420 |
Gene Ontology | GO:0016592; C:mediator complex; IDA:SGD. GO:0001105; F:RNA polymerase II transcription coactivator activity; IDA:SGD. GO:0001135; F:RNA polymerase II transcription factor recruiting transcription factor activity; IMP:SGD. GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD. GO:0000435; P:positive regulation of transcription from RNA polymerase II promoter by galactose; IMP:SGD. |
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