CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007697
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Uncharacterized peptidase YFR006W 
Protein Synonyms/Alias
  
Gene Name
 YFR006W 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
41GILQPEYKDKYYYSKubiquitination[1]
43LQPEYKDKYYYSKEKubiquitination[1]
50KYYYSKEKGEEMGEVubiquitination[1, 2, 3, 4, 5]
190SDLGKKFKELQDFAIubiquitination[1]
500EDDILVTKDGYENLTubiquitination[1]
518SDPDEIEKIVQKGLKubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [5] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
  
Sequence Annotation
 METAL 316 316 Manganese 2 (Potential).
 METAL 327 327 Manganese 1 (Potential).
 METAL 327 327 Manganese 2 (Potential).
 METAL 412 412 Manganese 1 (Potential).
 METAL 452 452 Manganese 1 (Potential).
 METAL 493 493 Manganese 1 (Potential).
 METAL 493 493 Manganese 2 (Potential).
 CROSSLNK 50 50 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Hydrolase; Isopeptide bond; Manganese; Membrane; Metal-binding; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 535 AA 
Protein Sequence
MCLEPISLVV FGSLVFFFGL VKYFKRGERQ RTRGILQPEY KDKYYYSKEK GEEMGEVANV 60
NEIPVKIRNH KYPAKEHNLR VKDLLLNRNP KLSKISTAFF IAGEELEGNK YCDTNKDFRQ 120
NRYFYHLSGV DIPASAILFN CSTDKLTLFL PNIDEEDVIW SGMPLSLDEA MRVFDIDEAL 180
YISDLGKKFK ELQDFAIFTT DLDNVHDENI ARSLIPSDPN FFYAMDETRA IKDWYEIESI 240
RKACQISDKS HLAVMSALPI ELNELQIQAE FEYHATRQGG RSLGYDPICC SGPACGTLHY 300
VKNSEDIKGK HSILIDAGAE WRQYTSDITR CFPTSGKFTA EHREVYETVL DMQNQAMERI 360
KPGAKWDDLH ALTHKVLIKH FLSMGIFKKE FSEDEIFKRR ASCAFYPHGL GHMLGLDVHD 420
VGGNPNYDDP DPMFRYLRIR RPLKENMVIT NEPGCYFNQF LIKEFLEKHP ERLEVVDMSV 480
LKRYMYVGGV RIEDDILVTK DGYENLTGIT SDPDEIEKIV QKGLKKPRSG FHVIV 535 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004177; F:aminopeptidase activity; IEA:InterPro.
 GO:0030145; F:manganese ion binding; IEA:InterPro.
 GO:0009987; P:cellular process; IEA:InterPro. 
Interpro
 IPR007865; Aminopep_P_N.
 IPR000994; Pept_M24_structural-domain.
 IPR001131; Peptidase_M24B_aminopep-P_CS. 
Pfam
 PF05195; AMP_N
 PF00557; Peptidase_M24 
SMART
 SM01011; AMP_N 
PROSITE
 PS00491; PROLINE_PEPTIDASE 
PRINTS