CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000762
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integral membrane protein 2A 
Protein Synonyms/Alias
 Protein E25 
Gene Name
 ITM2A 
Gene Synonyms/Alias
 UNQ603/PRO1189 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
3*****MVKIAFNTPTubiquitination[1, 2]
14NTPTAVQKEEARQDVubiquitination[1, 2, 3, 4, 5]
37RTQILTGKELRVATQubiquitination[1, 2, 3, 4, 5]
46LRVATQEKEGSSGRCubiquitination[5]
182NLVELFGKLASGRYLubiquitination[1]
238DLLLGFNKRAIDKCWubiquitination[1, 2]
243FNKRAIDKCWKIRHFubiquitination[1]
246RAIDKCWKIRHFPNEubiquitination[1]
259NEFIVETKICQE***ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
 DOMAIN 133 227 BRICHOS.
 CARBOHYD 166 166 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Glycoprotein; Membrane; Polymorphism; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 263 AA 
Protein Sequence
MVKIAFNTPT AVQKEEARQD VEALLSRTVR TQILTGKELR VATQEKEGSS GRCMLTLLGL 60
SFILAGLIVG GACIYKYFMP KSTIYRGEMC FFDSEDPANS LRGGEPNFLP VTEEADIRED 120
DNIAIIDVPV PSFSDSDPAA IIHDFEKGMT AYLDLLLGNC YLMPLNTSIV MPPKNLVELF 180
GKLASGRYLP QTYVVREDLV AVEEIRDVSN LGIFIYQLCN NRKSFRLRRR DLLLGFNKRA 240
IDKCWKIRHF PNEFIVETKI CQE 263 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. 
Interpro
 IPR007084; BRICHOS_dom. 
Pfam
 PF04089; BRICHOS 
SMART
 SM01039; BRICHOS 
PROSITE
 PS50869; BRICHOS 
PRINTS