CPLM-023621

Genbank Nucleotide ID

Integral membrane protein 2B

Protein Synonyms/Alias

Immature BRI2; imBRI2; Protein E25B; Transmembrane protein BRI; Bri; BRI2, membrane form; Mature BRI2; mBRI2; BRI2 intracellular domain; BRI2 ICD; BRI2C, soluble form; Bri23 peptide; Bri2-23; ABri23; C-terminal peptide; P23 peptide

Homo sapiens (Human)

Position	Peptide	Type	References
3	*****MVKVTFNSAL	ubiquitination	[1, 2]
13	FNSALAQKEAKKDEP	ubiquitination	[1, 2, 3, 4, 5, 6]
16	ALAQKEAKKDEPKSG	ubiquitination	[5]
17	LAQKEAKKDEPKSGE	ubiquitination	[5]
39	DAVAVDCKDPDDVVP	ubiquitination	[3, 7]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Plays a regulatory role in the processing of the beta- amyloid A4 precursor protein (APP) and acts as an inhibitor of the beta-amyloid peptide aggregation and fibrils deposition. Plays a role in the induction of neurite outgrowth. Functions as a protease inhibitor by blocking access of secretases to APP cleavage sites.

DOMAIN 137 231 BRICHOS.
REGION 102 134 Necessary for interaction with APP and
CARBOHYD 170 170 N-linked (GlcNAc...).
DISULFID 89 89 Interchain.
DISULFID 164 223 Probable.
DISULFID 248 265 Interchain (between ADan peptide

Amyloid; Amyloidosis; Cell membrane; Cleavage on pair of basic residues; Complete proteome; Deafness; Disease mutation; Disulfide bond; Endosome; Glycoprotein; Golgi apparatus; Membrane; Neurodegeneration; Reference proteome; Secreted; Signal-anchor; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
GO:0005615; C:extracellular space; IDA:UniProtKB.
GO:0030660; C:Golgi-associated vesicle membrane; IDA:UniProtKB.
GO:0031301; C:integral to organelle membrane; IDA:UniProtKB.
GO:0005634; C:nucleus; IDA:HPA.
GO:0005886; C:plasma membrane; IDA:UniProtKB.
GO:0005524; F:ATP binding; IEA:Compara.
GO:0001540; F:beta-amyloid binding; IPI:BHF-UCL.
GO:0006915; P:apoptotic process; IEA:Compara.
GO:0006917; P:induction of apoptosis; IEA:Compara.
GO:0042985; P:negative regulation of amyloid precursor protein biosynthetic process; IDA:UniProtKB.
GO:0007399; P:nervous system development; TAS:ProtInc.

IPR007084; BRICHOS_dom.