CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018237
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 473 
Protein Synonyms/Alias
 Zinc finger protein 100 homolog; Zfp-100 
Gene Name
 ZNF473 
Gene Synonyms/Alias
 KIAA1141; ZFP100 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
235WITHTREKPTVHQECubiquitination[1]
312TQPGEHQKTHTDSKSubiquitination[2]
382SECQECGKIFRHSSLubiquitination[1]
485QAIHTAEKPYSCAECubiquitination[1]
493PYSCAECKETFSDNNubiquitination[1]
512HQKMHTVKTPYECQEubiquitination[1]
541ESVHAREKQGFFVSGubiquitination[1]
558LDQNPEQKEKCFKCNubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases. 
Sequence Annotation
 DOMAIN 6 75 KRAB.
 ZN_FING 209 231 C2H2-type 1.
 ZN_FING 265 286 C2H2-type 2; degenerate.
 ZN_FING 320 342 C2H2-type 3.
 ZN_FING 347 369 C2H2-type 4.
 ZN_FING 375 397 C2H2-type 5.
 ZN_FING 403 425 C2H2-type 6; degenerate.
 ZN_FING 431 453 C2H2-type 7.
 ZN_FING 459 481 C2H2-type 8.
 ZN_FING 487 509 C2H2-type 9.
 ZN_FING 515 537 C2H2-type 10.
 ZN_FING 562 584 C2H2-type 11.
 ZN_FING 591 613 C2H2-type 12.
 ZN_FING 646 668 C2H2-type 13.
 ZN_FING 674 696 C2H2-type 14.
 ZN_FING 702 724 C2H2-type 15.
 ZN_FING 730 752 C2H2-type 16.
 ZN_FING 758 780 C2H2-type 17.
 ZN_FING 786 808 C2H2-type 18.
 ZN_FING 814 836 C2H2-type 19.
 ZN_FING 842 864 C2H2-type 20.
 REGION 312 552 Interaction with SLBP/pre-mRNA complex.  
Keyword
 3D-structure; Complete proteome; DNA-binding; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 871 AA 
Protein Sequence
MAEEFVTLKD VGMDFTLGDW EQLGLEQGDT FWDTALDNCQ DLFLLDPPRP NLTSHPDGSE 60
DLEPLAGGSP EATSPDVTET KNSPLMEDFF EEGFSQEIIE MLSKDGFWNS NFGEACIEDT 120
WLDSLLGDPE SLLRSDIATN GESPTECKSH ELKRGLSPVS TVSTGEDSMV HNVSEKTLTP 180
AKSKEYRGEF FSYSDHSQQD SVQEGEKPYQ CSECGKSFSG SYRLTQHWIT HTREKPTVHQ 240
ECEQGFDRNA SLSVYPKTHT GYKFYVCNEY GTTFSQSTYL WHQKTHTGEK PCKSQDSDHP 300
PSHDTQPGEH QKTHTDSKSY NCNECGKAFT RIFHLTRHQK IHTRKRYECS KCQATFNLRK 360
HLIQHQKTHA AKTTSECQEC GKIFRHSSLL IEHQALHAGE EPYKCNERGK SFRHNSTLKI 420
HQRVHSGEKP YKCSECGKAF HRHTHLNEHR RIHTGYRPHK CQECVRSFSR PSHLMRHQAI 480
HTAEKPYSCA ECKETFSDNN RLVQHQKMHT VKTPYECQEC GERFICGSTL KCHESVHARE 540
KQGFFVSGKI LDQNPEQKEK CFKCNKCEKT FSCSKYLTQH ERIHTRGVKP FECDQCGKAF 600
GQSTRLIHHQ RIHSRVRLYK WGEQGKAISS ASLIKLQSFH TKEHPFKCNE CGKTFSHSAH 660
LSKHQLIHAG ENPFKCSKCD RVFTQRNYLV QHERTHARKK PLVCNECGKT FRQSSCLSKH 720
QRIHSGEKPY VCDYCGKAFG LSAELVRHQR IHTGEKPYVC QECGKAFTQS SCLSIHRRVH 780
TGEKPYRCGE CGKAFAQKAN LTQHQRIHTG EKPYSCNVCG KAFVLSAHLN QHLRVHTQET 840
LYQCQRCQKA FRCHSSLSRH QRVHNKQQYC L 871 
Gene Ontology
 GO:0015030; C:Cajal body; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006398; P:histone mRNA 3'-end processing; IDA:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB
 PF00096; zf-C2H2 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS