CPLM-011397

Genbank Nucleotide ID

Transaminated amino acid decarboxylase

Protein Synonyms/Alias

Transaminated branched-chain amino acid decarboxylase

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
33	PFGEYIFKRLLSIDT	acetylation	[1]
129	LHIVGVAKSIDSRSS	acetylation	[1]
168	KVYHDMVKDRVACSV	acetylation	[1]
372	QGNEQMFKGINFAPI	ubiquitination	[2]
588	TLIQCPSKLALKLEE	acetylation	[1]
588	TLIQCPSKLALKLEE	ubiquitination	[3, 4, 5]

[1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
[2] Systematic approach for validating the ubiquitinated proteome.
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149]
[3] A proteomics approach to understanding protein ubiquitination.
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
[4] Computational identification of ubiquitylation sites from protein sequences.
Tung CW, Ho SY.
BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
[5] Identification, analysis, and prediction of protein ubiquitination sites.
Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]

Functional Description

One of five 2-oxo acid decarboxylases (PDC1, PDC5, PDC6, ARO10, and THI3) involved in amino acid catabolism. The enzyme catalyzes the decarboxylation of amino acids, which, in a first step, have been transaminated to the corresponding 2-oxo acids (alpha-keto-acids). In a third step, the resulting aldehydes are reduced to alcohols, collectively referred to as fusel oils or alcohols. Its preferred substrates are the transaminated amino acids, phenylalanine, tryptophan, (and probably tyrosine), but also isoleucine, whereas leucine is a low efficiency and valine and pyruvate are no substrates. In analogy to the pyruvate decarboxylases the enzyme may in a side-reaction catalyze condensation (or carboligation) reactions leading to the formation of 2-hydroxy ketone, collectively called acyloins.

CROSSLNK 588 588 Glycyl lysine isopeptide (Lys-Gly)

Branched-chain amino acid catabolism; Complete proteome; Cytoplasm; Decarboxylase; Isopeptide bond; Lyase; Magnesium; Metal-binding; Phenylalanine catabolism; Reference proteome; Thiamine pyrophosphate; Tryptophan catabolism; Tyrosine catabolism; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:SGD.
GO:0000287; F:magnesium ion binding; IEA:InterPro.
GO:0050177; F:phenylpyruvate decarboxylase activity; IDA:SGD.
GO:0004737; F:pyruvate decarboxylase activity; ISS:SGD.
GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
GO:0000949; P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway; IGI:SGD.
GO:0000950; P:branched-chain amino acid catabolic process to alcohol via Ehrlich pathway; IGI:SGD.
GO:0006559; P:L-phenylalanine catabolic process; IDA:SGD.
GO:0006552; P:leucine catabolic process; IMP:SGD.
GO:0000951; P:methionine catabolic process to 3-methylthiopropanol; IMP:SGD.
GO:0006569; P:tryptophan catabolic process; IGI:SGD.
GO:0006572; P:tyrosine catabolic process; IEA:UniProtKB-KW.

IPR012000; Thiamin_PyroP_enz_cen_dom.
IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
IPR012110; TPP_enzyme.
IPR011766; TPP_enzyme-bd_C.

PF02775; TPP_enzyme_C
PF00205; TPP_enzyme_M
PF02776; TPP_enzyme_N