UniProt Accession

 ICLN_HUMAN; P54105; B2RCS9; Q0VDK6; Q9NRD2; Q9NRD3 

Genbank Protein ID

 X91788; U17899; U53454; AF005422; AF026003; AF232708; AF232224; AF232225; AK315259; BT019907; BC119634; BC119635 

Genbank Nucleotide ID

 CAA62902.1; AAC50111.1; AAB03316.1; AAB61444.1; AAB88806.1; AAF76861.1; AAF76858.1; AAF76859.1; BAG37676.1; AAV38710.1; AAI19635.1; AAI19636.1 

Protein Name

 Methylosome subunit pICln 

Protein Synonyms/Alias

 Chloride channel, nucleotide sensitive 1A; Chloride conductance regulatory protein ICln; I(Cln); Chloride ion current inducer protein; ClCI; Reticulocyte pICln 

Gene Name

 CLNS1A 

Gene Synonyms/Alias

 CLCI; ICLN 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MSFLKSFPPPGP	ubiquitination	[1, 2, 3]
30	TEAVLNGKGLGTGTL	ubiquitination	[1, 3, 4, 5, 6]
91	AKFEEESKEPVADEE	ubiquitination	[4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 The interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.
 MOD_RES 102 102 Phosphoserine.
 MOD_RES 223 223 Phosphothreonine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 237 AA 

Protein Sequence

Gene Ontology

 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:ProtInc.
 GO:0008015; P:blood circulation; TAS:ProtInc.
 GO:0006884; P:cell volume homeostasis; IEA:InterPro.
 GO:0006821; P:chloride transport; IEA:InterPro.
 GO:0034660; P:ncRNA metabolic process; TAS:Reactome.
 GO:0000387; P:spliceosomal snRNP assembly; TAS:Reactome.
 GO:0006810; P:transport; TAS:ProtInc. 

Interpro

 IPR003521; ICln_channel. 

Pfam

  

SMART

  

PROSITE

  

PRINTS

 PR01348; ICLNCHANNEL.