| Tag | Content |
|---|
| CPLM ID | CPLM-024235 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Proline--tRNA ligase |
Protein Synonyms/Alias | Prolyl-tRNA synthetase; ProRS |
Gene Name | proS |
Gene Synonyms/Alias | MSMEG_2621; MSMEI_2559 |
Created Date | July 27, 2013 |
Organism | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) |
NCBI Taxa ID | 246196 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 173 | DVDDDGLKNAYYQHR | pupylation | [1] |
|
Reference | [1] Expansion of the mycobacterial "PUPylome". Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC. Mol Biosyst. 2010 Feb;6(2):376-85. [ PMID: 20094657] |
Functional Description | Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS (By similarity). |
Sequence Annotation | CROSSLNK 173 173 Isoglutamyl lysine isopeptide (Lys-Gln) |
Keyword | Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; Ubl conjugation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 585 AA |
Protein Sequence | MITRMSELFL RTLRDDPADA EVPSHKLLIR AGYVRAVGPG IYSWLPLGLR VLRKIENVVR 60
SEMNAIGAQE ILLPALLPRG PYETTNRWTE YGDTLFRLQD RRNNDYLLGP THEELFTLTV 120
KGEYSSYKDF PVILYQIQTK YRDEARPRAG ILRGREFVMK DSYSFDVDDD GLKNAYYQHR 180
EAYQRIFARL GVRYVIVSAV SGAMGGSASE EFLAESEVGE DTFVRCVESG YAANVEAVIT 240
RAPEAQPTEG LPEAKVYDTP DTPTIATLVE WANSASLPQF EGRTVTAADT LKNVLLKTRE 300
PGGEWELLAV GVPGDREVDE KRLGAALEPA EFALLDDADF AANPFLVKGY VGPKALQDNG 360
VRYLVDPRVV HGSSWITGAD APNRHVVGLV AGRDFTPDGT IEAAEVRDGD PSPDGAGVLT 420
SARGIEIGHI FQLGRKYTDA FSADVLGEDG KPLRLTMGSY GIGVSRLVAV IAEQQHDQLG 480
LRWPSSVAPF DVHVVVANKD AGARAGAAEL VADLDRLGHE VLFDDRQASP GVKFKDAELL 540
GMPWIVVVGR GWADGVVELR NRFTGETREI AADGAAAEIS SVLAG 585 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004827; F:proline-tRNA ligase activity; IEA:EC. GO:0006433; P:prolyl-tRNA aminoacylation; IEA:InterPro. GO:0006450; P:regulation of translational fidelity; IEA:GOC. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |