CPLM-021299

Genbank Nucleotide ID

Enhancer of polycomb homolog 1

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
294	MAQRQPMKPTYAIPI	acetylation	[1]
512	TSDKSFSKDLSQILV	acetylation	[2, 3]
522	SQILVNIKSCRWRHF	ubiquitination	[4, 5]

[1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage.

Activator; Alternative splicing; Chromatin regulator; Complete proteome; Direct protein sequencing; Growth regulation; Nucleus; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0031965; C:nuclear membrane; IDA:MGI.
GO:0032777; C:Piccolo NuA4 histone acetyltransferase complex; IDA:UniProtKB.
GO:0043968; P:histone H2A acetylation; IDA:UniProtKB.
GO:0043967; P:histone H4 acetylation; IDA:UniProtKB.
GO:0045814; P:negative regulation of gene expression, epigenetic; IDA:MGI.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
GO:0006351; P:transcription, DNA-dependent; TAS:UniProtKB.

IPR024943; Enhancer_polycomb.
IPR019542; Enhancer_polycomb-like_N.
IPR009607; Enhancer_polycomb_C.

PF06752; E_Pc_C
PF10513; EPL1