CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002253
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor AP-1 
Protein Synonyms/Alias
 Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 
Gene Name
 JUN 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50ADPVGSLKPHLRAKNacetylation[1, 2]
50ADPVGSLKPHLRAKNubiquitination[3, 4, 5]
268RNRIAASKCRKRKLEacetylation[6]
271IAASKCRKRKLERIAacetylation[6]
273ASKCRKRKLERIARLacetylation[6]
Reference
 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Activation of extracellular signal-regulated kinase signaling by epidermal growth factor mediates c-Jun activation and p300 recruitment in keratin 16 gene expression.
 Wang YN, Chen YJ, Chang WC.
 Mol Pharmacol. 2006 Jan;69(1):85-98. [PMID: 16214953] 
Functional Description
 Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. 
Sequence Annotation
 DOMAIN 252 315 bZIP.
 REGION 252 279 Basic motif (By similarity).
 REGION 280 308 Leucine-zipper (By similarity).
 MOD_RES 2 2 Phosphothreonine; by PAK2.
 MOD_RES 8 8 Phosphothreonine; by PAK2.
 MOD_RES 58 58 Phosphoserine.
 MOD_RES 63 63 Phosphoserine; by MAPK8 and PLK3.
 MOD_RES 73 73 Phosphoserine; by MAPK8 and PLK3.
 MOD_RES 89 89 Phosphothreonine; by PAK2.
 MOD_RES 93 93 Phosphothreonine; by PAK2.
 MOD_RES 239 239 Phosphothreonine; by GSK3-beta.
 MOD_RES 243 243 Phosphoserine; by DYRK2 and GSK3-beta.
 MOD_RES 249 249 Phosphoserine; by GSK3-beta.
 MOD_RES 271 271 N6-acetyllysine.
 MOD_RES 286 286 Phosphothreonine; by PAK2.  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 331 AA 
Protein Sequence
MTAKMETTFY DDALNASFLP SESGPYGYSN PKILKQSMTL NLADPVGSLK PHLRAKNSDL 60
LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP KNVTDEQEGF AEGFVRALAE 120
LHSQNTLPSV TSAAQPVNGA GMVAPAVASV AGGSGSGGFS ASLHSEPPVY ANLSNFNPGA 180
LSSGGGAPSY GAAGLAFPAQ PQQQQQPPHH LPQQMPVQHP RLQALKEEPQ TVPEMPGETP 240
PLSPIDMESQ ERIKAERKRM RNRIAASKCR KRKLERIARL EEKVKTLKAQ NSELASTANM 300
LREQVAQLKQ KVMNHVNSGC QLMLTQQLQT F 331 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005719; C:nuclear euchromatin; IDA:BHF-UCL.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0017053; C:transcriptional repressor complex; IEA:Compara.
 GO:0035497; F:cAMP response element binding; IDA:BHF-UCL.
 GO:0003690; F:double-stranded DNA binding; IEA:Compara.
 GO:0005100; F:Rho GTPase activator activity; IDA:UniProtKB.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0000980; F:RNA polymerase II distal enhancer sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; IC:BHF-UCL.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0007568; P:aging; IEA:Compara.
 GO:0001525; P:angiogenesis; IEA:Compara.
 GO:0031103; P:axon regeneration; IEA:Compara.
 GO:0071277; P:cellular response to calcium ion; IEA:Compara.
 GO:0051365; P:cellular response to potassium ion starvation; IEA:Compara.
 GO:0007623; P:circadian rhythm; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0035026; P:leading edge cell differentiation; IEA:Compara.
 GO:0007612; P:learning; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0051899; P:membrane depolarization; IEA:Compara.
 GO:0001774; P:microglial cell activation; IEA:Compara.
 GO:0030224; P:monocyte differentiation; IEA:Compara.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0043922; P:negative regulation by host of viral transcription; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0043392; P:negative regulation of DNA binding; IDA:UniProtKB.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0031953; P:negative regulation of protein autophosphorylation; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0003151; P:outflow tract morphogenesis; IEA:Compara.
 GO:0043923; P:positive regulation by host of viral transcription; IDA:UniProtKB.
 GO:0045740; P:positive regulation of DNA replication; IEA:Compara.
 GO:0001938; P:positive regulation of endothelial cell proliferation; IEA:Compara.
 GO:0048146; P:positive regulation of fibroblast proliferation; IEA:Compara.
 GO:0045657; P:positive regulation of monocyte differentiation; IEA:Compara.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0051726; P:regulation of cell cycle; IEA:Compara.
 GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; TAS:Reactome.
 GO:0001836; P:release of cytochrome c from mitochondria; IEA:Compara.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0034097; P:response to cytokine stimulus; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0042542; P:response to hydrogen peroxide; IEA:Compara.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0009612; P:response to mechanical stimulus; IEA:Compara.
 GO:0009314; P:response to radiation; IEA:Compara.
 GO:0007184; P:SMAD protein import into nucleus; IDA:BHF-UCL.
 GO:0060395; P:SMAD protein signal transduction; IDA:BHF-UCL.
 GO:0051403; P:stress-activated MAPK cascade; TAS:Reactome.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; IDA:BHF-UCL.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR004827; bZIP.
 IPR015558; C_Jun.
 IPR008917; Euk_TF_DNA-bd.
 IPR005643; JNK.
 IPR002112; Leuzip_Jun. 
Pfam
 PF00170; bZIP_1
 PF03957; Jun 
SMART
 SM00338; BRLZ 
PROSITE
 PS50217; BZIP
 PS00036; BZIP_BASIC 
PRINTS
 PR00043; LEUZIPPRJUN.