CPLM-015773

Genbank Nucleotide ID

Lysophosphatidylcholine acyltransferase 2

Protein Synonyms/Alias

LPC acyltransferase 2; LPCAT-2; LysoPC acyltransferase 2; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 1

Homo sapiens (Human)

Position	Peptide	Type	References
204	NTINEIIKRTTSGGE	ubiquitination	[1, 2]
375	ASIASSSKGGRIGIE	ubiquitination	[1, 3]
386	IGIEEFAKYLKLPVS	ubiquitination	[4, 5]
389	EEFAKYLKLPVSDVL	ubiquitination	[4, 5]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet- activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso- PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases (By similarity).

DOMAIN 391 426 EF-hand 1.
DOMAIN 428 463 EF-hand 2.
MOTIF 146 151 HXXXXD motif.

Acyltransferase; Alternative splicing; Calcium; Complete proteome; Endoplasmic reticulum; Golgi apparatus; Lipid biosynthesis; Lipid metabolism; Membrane; Metal-binding; Phospholipid biosynthesis; Phospholipid metabolism; Polymorphism; Reference proteome; Repeat; Signal-anchor; Transferase; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
GO:0005795; C:Golgi stack; ISS:UniProtKB.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0047184; F:1-acylglycerophosphocholine O-acyltransferase activity; ISS:UniProtKB.
GO:0047192; F:1-alkylglycerophosphocholine O-acetyltransferase activity; ISS:UniProtKB.
GO:0005509; F:calcium ion binding; IEA:InterPro.
GO:0016044; P:cellular membrane organization; ISS:UniProtKB.
GO:0036151; P:phosphatidylcholine acyl-chain remodeling; TAS:Reactome.
GO:0006663; P:platelet activating factor biosynthetic process; ISS:UniProtKB.

IPR011992; EF-hand-like_dom.
IPR018247; EF_Hand_1_Ca_BS.
IPR002048; EF_hand_dom.
IPR002123; Plipid/glycerol_acylTrfase.
IPR001125; Recoverin.

PF01553; Acyltransferase
PF13499; EF_hand_5

SM00054; EFh
SM00563; PlsC

PS00018; EF_HAND_1
PS50222; EF_HAND_2