CPLM-006445

Genbank Nucleotide ID

Polycomb group RING finger protein 2

Protein Synonyms/Alias

DNA-binding protein Mel-18; RING finger protein 110; Zinc finger protein 144

MEL18; RNF110; ZNF144

Homo sapiens (Human)

Position	Peptide	Type	References
73	LLSIRSDKTLQDIVY	ubiquitination	[1, 2, 3]
226	RNGPLPLKYRVQPAC	ubiquitination	[2]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero- posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with BMI1; unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex (By similarity).

ZN_FING 18 57 RING-type.
MOTIF 81 95 Nuclear localization signal (Potential).
MOD_RES 344 344 Phosphothreonine.

Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016604; C:nuclear body; IEA:Compara.
GO:0000790; C:nuclear chromatin; IDA:UniProtKB.
GO:0035102; C:PRC1 complex; IDA:UniProtKB.
GO:0001739; C:sex chromatin; IEA:Compara.
GO:0003682; F:chromatin binding; IEA:Compara.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0009952; P:anterior/posterior pattern specification; IEA:Compara.
GO:0048704; P:embryonic skeletal system morphogenesis; IEA:Compara.
GO:0016573; P:histone acetylation; IEA:Compara.
GO:0001701; P:in utero embryonic development; IEA:Compara.
GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
GO:0016567; P:protein ubiquitination; IEA:GOC.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR018957; Znf_C3HC4_RING-type.
IPR001841; Znf_RING.
IPR013083; Znf_RING/FYVE/PHD.
IPR017907; Znf_RING_CS.

PS00518; ZF_RING_1
PS50089; ZF_RING_2