CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012556
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras suppressor protein 1 
Protein Synonyms/Alias
 RSP-1; Rsu-1 
Gene Name
 RSU1 
Gene Synonyms/Alias
 RSP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MSKSLKKLVEESRubiquitination[1]
7*MSKSLKKLVEESREubiquitination[1]
89ISSLQKLKHLNLGMNubiquitination[2]
155ILPPDIGKLTKLQILubiquitination[3, 4]
158PDIGKLTKLQILSLRubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
183IGELTQLKELHIQGNubiquitination[1, 2, 3, 4, 6, 7]
208NLDLTGQKQVFKAENubiquitination[1, 2, 3, 4]
243YIRSETYKYLYGRHMubiquitination[2, 7]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Potentially plays a role in the Ras signal transduction pathway. Capable of suppressing v-Ras transformation in vitro. 
Sequence Annotation
 REPEAT 41 63 LRR 1.
 REPEAT 64 85 LRR 2.
 REPEAT 87 109 LRR 3.
 REPEAT 110 133 LRR 4.
 REPEAT 135 156 LRR 5.
 REPEAT 158 179 LRR 6.
 REPEAT 181 202 LRR 7.  
Keyword
 Alternative splicing; Complete proteome; Direct protein sequencing; Leucine-rich repeat; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 277 AA 
Protein Sequence
MSKSLKKLVE ESREKNQPEV DMSDRGISNM LDVNGLFTLS HITQLVLSHN KLTMVPPNIA 60
ELKNLEVLNF FNNQIEELPT QISSLQKLKH LNLGMNRLNT LPRGFGSLPA LEVLDLTYNN 120
LSENSLPGNF FYLTTLRALY LSDNDFEILP PDIGKLTKLQ ILSLRDNDLI SLPKEIGELT 180
QLKELHIQGN RLTVLPPELG NLDLTGQKQV FKAENNPWVT PIADQFQLGV SHVFEYIRSE 240
TYKYLYGRHM QANPEPPKKN NDKSKKISRK PLAAKNR 277 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0034329; P:cell junction assembly; TAS:Reactome.
 GO:0007265; P:Ras protein signal transduction; IEA:Compara.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR025875; Leu-rich_rpt_4.
 IPR003591; Leu-rich_rpt_typical-subtyp. 
Pfam
 PF00560; LRR_1
 PF12799; LRR_4 
SMART
 SM00369; LRR_TYP 
PROSITE
 PS51450; LRR 
PRINTS