CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023744
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Endophilin-B1 
Protein Synonyms/Alias
 Bax-interacting factor 1; Bif-1; SH3 domain-containing GRB2-like protein B1 
Gene Name
 SH3GLB1 
Gene Synonyms/Alias
 KIAA0491; CGI-61 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9NIMDFNVKKLAADAGubiquitination[1]
10IMDFNVKKLAADAGTubiquitination[1]
29AVQFTEEKLGQAEKTubiquitination[1]
35EKLGQAEKTELDAHLubiquitination[1]
48HLENLLSKAECTKIWubiquitination[1]
53LSKAECTKIWTEKIMubiquitination[1]
58CTKIWTEKIMKQTEVubiquitination[1]
61IWTEKIMKQTEVLLQubiquitination[1]
82IEEFVYEKLDRKAPSubiquitination[1, 2, 3]
156NFIEGDYKTIAKERKubiquitination[1]
176RLDLDAAKTRLKKAKubiquitination[1]
376ERGNQKGKVPITYLEubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. 
Sequence Annotation
 DOMAIN 27 261 BAR.
 DOMAIN 305 365 SH3.
 REGION 1 30 Membrane-binding amphipathic helix.
 MOD_RES 145 145 Phosphothreonine; by CDK5.  
Keyword
 Alternative splicing; Apoptosis; Coiled coil; Complete proteome; Cytoplasm; Golgi apparatus; Lipid-binding; Membrane; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 365 AA 
Protein Sequence
MNIMDFNVKK LAADAGTFLS RAVQFTEEKL GQAEKTELDA HLENLLSKAE CTKIWTEKIM 60
KQTEVLLQPN PNARIEEFVY EKLDRKAPSR INNPELLGQY MIDAGTEFGP GTAYGNALIK 120
CGETQKRIGT ADRELIQTSA LNFLTPLRNF IEGDYKTIAK ERKLLQNKRL DLDAAKTRLK 180
KAKAAETRNS QLNSARLEGD NIMIWAEEVT KSEQELRITQ SEFDRQAEIT RLLLEGISST 240
HAHHLRCLND FVEAQMTYYA QCYQYMLDLQ KQLGSFPSNY LSNNNQTSVT PVPSVLPNAI 300
GSSAMASTSG LVITSPSNLS DLKECSGSRK ARVLYDYDAA NSTELSLLAD EVITVFSVVG 360
MDSDWLMGER GNQKGKVPIT YLELLN 386 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HGNC.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0005504; F:fatty acid binding; IEA:Compara.
 GO:0042171; F:lysophosphatidic acid acyltransferase activity; IEA:Compara.
 GO:0051084; P:'de novo' posttranslational protein folding; IEA:Compara.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IDA:HGNC.
 GO:0006654; P:phosphatidic acid biosynthetic process; IEA:Compara.
 GO:1900740; P:positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; IEA:Compara.
 GO:0032461; P:positive regulation of protein oligomerization; IDA:UniProtKB.
 GO:0051259; P:protein oligomerization; IDA:UniProtKB. 
Interpro
 IPR027267; AH/BAR-dom.
 IPR004148; BAR_dom.
 IPR001452; SH3_domain. 
Pfam
 PF03114; BAR
 PF00018; SH3_1 
SMART
 SM00721; BAR
 SM00326; SH3 
PROSITE
 PS51021; BAR
 PS50002; SH3 
PRINTS