CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033057
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Regulator of chromosome condensation 
Protein Synonyms/Alias
  
Gene Name
 RCC1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
122GSEMVPGKVELQEKVubiquitination[1, 2]
128GKVELQEKVVQVSAGubiquitination[3]
196IGLLEPMKKSMVPVQubiquitination[2, 4]
197GLLEPMKKSMVPVQVubiquitination[2, 5, 6]
263LERLLVPKCVMLKSRubiquitination[2]
268VPKCVMLKSRGSR**ubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 273 AA 
Protein Sequence
MMEAEDRKMS PKRIAKRRSP PADAIPKSKK VKVSHRSHST EPGLVLTLGQ GDVGQLGLGE 60
NVMERKKPAL VSIPEDVVQA EAGGMHTVCL SKSGQVYSFG CNDEGALGRD TSVEGSEMVP 120
GKVELQEKVV QVSAGDSHTA ALTDDGRVFL WGSFRHCLFH TSQRLGWGKE VWRQGLSHLR 180
VSKDNNGVIG LLEPMKKSMV PVQVQLDVPV VKVASGNDHL VMLTADGDLY TLGCGEQGQL 240
GRVPELFANR GGRQGLERLL VPKCVMLKSR GSR 273 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA. 
Interpro
 IPR009091; RCC1/BLIP-II.
 IPR000408; Reg_chr_condens. 
Pfam
 PF00415; RCC1 
SMART
  
PROSITE
 PS00625; RCC1_1
 PS00626; RCC1_2
 PS50012; RCC1_3 
PRINTS
 PR00633; RCCNDNSATION.