CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037271
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Hexokinase-2 
Protein Synonyms/Alias
  
Gene Name
 HK2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13ETLLEISKRFRKEMEubiquitination[1, 2, 3]
21RFRKEMEKGLGATTHubiquitination[2]
34THPTAAVKMLPTFVRubiquitination[2, 3, 4]
76VTDNGLQKVEMENQIubiquitination[1, 2, 5]
119KLQIKDKKLPLGFTFubiquitination[2]
145SFLVSWTKGFKSSGVubiquitination[1, 2, 3, 5]
148VSWTKGFKSSGVEGRubiquitination[2, 5]
262MGSLNPGKQLFEKMIubiquitination[1]
295EELLFGGKLSPELLNubiquitination[1, 2, 3, 4, 5]
309NTGRFETKDISDIEGubiquitination[1, 2, 5]
318ISDIEGEKDGIRKARubiquitination[2, 3]
390GVDGSVYKKHPHFAKubiquitination[1, 2]
391VDGSVYKKHPHFAKRubiquitination[2]
423RSEDGSGKGAAMVTAubiquitination[1, 2, 3]
444DQHRARQKTLEHLQLubiquitination[2]
460HDQLLEVKRRMKVEMubiquitination[1, 2, 3, 5]
464LEVKRRMKVEMERGLubiquitination[2]
473EMERGLSKETHASAPubiquitination[2]
596LKWTKGFKASGCEGEubiquitination[2]
610EDVVTLLKEAIHRREubiquitination[2, 3]
710ELSLNPGKQRFEKMIubiquitination[1]
715PGKQRFEKMISGMYLubiquitination[2]
735NILIDFTKRGLLFRGubiquitination[2, 5]
838GVDGTLYKLHPHFAKubiquitination[1, 2]
857TVKDLAPKCDVSFLQubiquitination[2]
871QSEDGSGKGAALITAubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 889 AA 
Protein Sequence
MRLSDETLLE ISKRFRKEME KGLGATTHPT AAVKMLPTFV RSTPDGTEHG EFLALDLGGT 60
NFRVLWVKVT DNGLQKVEME NQIYAIPEDI MRGSGTQLFD HIAECLANFM DKLQIKDKKL 120
PLGFTFSFPC HQTKLDESFL VSWTKGFKSS GVEGRDVVAL IRKAIQRRGD FDIDIVAVVN 180
DTVGTMMTCG YDDHNCEIGL IVGTGSNACY MEEMRHIDMV EGDEGRMCIN MEWGAFGDDG 240
SLNDIRTEFD QEIDMGSLNP GKQLFEKMIS GMYMGELVRL ILVKMAKEEL LFGGKLSPEL 300
LNTGRFETKD ISDIEGEKDG IRKAREVLMR LGLDPTQEDC VATHRICQIV STRSASLCAA 360
TLAAVLQRIK ENKGEERLRS TIGVDGSVYK KHPHFAKRLH KTVRRLVPGC DVRFLRSEDG 420
SGKGAAMVTA VAYRLADQHR ARQKTLEHLQ LSHDQLLEVK RRMKVEMERG LSKETHASAP 480
VKMLPTYVCA TPDGTEKGDF LALDLGGTNF RVLLVRVRNG KWGGVEMHNK IYAIPQEVMH 540
GTGDELFDHI VQCIADFLEY MGMKGVSLPL GFTFSFPCQQ NSLDESILLK WTKGFKASGC 600
EGEDVVTLLK EAIHRREEFD LDVVAVVNDT VGTMMTCGFE DPHCEVGLIV GTGSNACYME 660
EMRNVELVEG EEGRMCVNME WGAFGDNGCL DDFRTEFDVA VDELSLNPGK QRFEKMISGM 720
YLGEIVRNIL IDFTKRGLLF RGRISERLKT RGIFETKFLS QIESDCLALL QVRAILQHLG 780
LESTCDDSII VKEVCTVVAR RAAQLCGAGM AAVVDRIREN RGLDALKVTV GVDGTLYKLH 840
PHFAKVMHET VKDLAPKCDV SFLQSEDGSG KGAALITAVA CRIREAGQR 889 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005536; F:glucose binding; IEA:Compara.
 GO:0004396; F:hexokinase activity; IEA:Compara.
 GO:0008637; P:apoptotic mitochondrial changes; IEA:Compara.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0046324; P:regulation of glucose import; IEA:Compara. 
Interpro
 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 
Pfam
 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 
SMART
  
PROSITE
 PS00378; HEXOKINASES 
PRINTS
 PR00475; HEXOKINASE.