CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020409
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative nascent polypeptide-associated complex subunit alpha-like protein 
Protein Synonyms/Alias
 Alpha-NAC pseudogene 1; NAC-alpha pseudogene 1 
Gene Name
 NACAP1 
Gene Synonyms/Alias
 FKSG17 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99RVTIRKSKNILFVITubiquitination[1, 2]
107NILFVITKPDVYKSPubiquitination[1, 2]
141AQLAAAEKFKVQGEAacetylation[3]
141AQLAAAEKFKVQGEAubiquitination[1]
199AKAVRALKNSNDIVNubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
 DOMAIN 69 134 NAC-A/B.
 DOMAIN 175 211 UBA.
 MOD_RES 43 43 Phosphoserine (By similarity).
 MOD_RES 158 158 Phosphothreonine (By similarity).
 MOD_RES 160 160 Phosphothreonine (By similarity).
 MOD_RES 165 165 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 213 AA 
Protein Sequence
MPGEATETVP AIEQQLLQPQ AETGSGTESD SDESVPELEE QDSTQVTAQV QLVVAAEIDE 60
EPVSKAKQRR SEKKARKARF KLGLQQVTGV TRVTIRKSKN ILFVITKPDV YKSPASDTYM 120
VFGEAKIEDL SQEAQLAAAE KFKVQGEAVS NIQENTQTPT VQEGSEDEEV DETGVEIKDI 180
ELVLSQANVW GAKAVRALKN SNDIVNAIME LTM 213 
Gene Ontology
  
Interpro
 IPR016641; EGD2/NACA.
 IPR002715; Nas_poly-pep-assoc_cplx_dom. 
Pfam
 PF01849; NAC 
SMART
  
PROSITE
 PS51151; NAC_AB
 PS50030; UBA 
PRINTS