| Tag | Content |
|---|
| CPLM ID | CPLM-006171 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 4 |
Protein Synonyms/Alias | Deubiquitinating enzyme 4; Ubiquitin thioesterase 4; Ubiquitin-specific-processing protease 4; Vacuole biogenesis protein SSV7 |
Gene Name | DOA4 |
Gene Synonyms/Alias | DOS1; MUT4; NPI2; SSV7; UBP4; YDR069C; D4270; YD8554.02C; YD9609.23C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 44 | FIITGKAKDQDLSSL | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Involved in the ammonium-induced down-regulation of the GAP1 permease and the UME3 destruction in response to oxidative stress. Has a role in the RAD9 checkpoint response to TOP1 poisons. Required for promoting coordination of DNA replication and avoids DNA overreplication. |
Sequence Annotation | DOMAIN 205 328 Rhodanese.
ACT_SITE 571 571 Nucleophile.
ACT_SITE 880 880 Proton acceptor (By similarity).
MOD_RES 443 443 Phosphoserine. |
Keyword | Complete proteome; Cytoplasm; Endosome; Hydrolase; Membrane; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 926 AA |
Protein Sequence | MEQNIISTIR DECIRHRSKY LTIAQLTAIA EAKINEFIIT GKAKDQDLSS LLDKCIDILS 60
IYKKNSKDIK NIISCKNKGA MISSNSVMII QLNYVYYKVI HIIVTTNIPH LSEFAKIKLH 120
KSTSDEGNGN NNNNEFQLMN IYNTLLETLL KDENIAKIKS FIKSSIKQTK LNHEQEECNL 180
MRTGSYITSN QLNSLISSSA NSASSQMEIL LIDIRSRLEF NKSHIDTKNI ICLEPISFKM 240
SYSDHDLEKK SLITSPNSEI KMFQSRNLFK FIILYTDANE YNVKQQSVLL DILVNHSFEK 300
PISDDFTKIF ILESGFPGWL KSNYGRQVSS SFPSNNNIKD DSVYINGNTS GLSLQHLPKM 360
SPSIRHSMDD SMKEMLVAPT PLNHLQQQQQ QQSDNDHVLK RSSSFKKLFS NYTSPNPKNS 420
NSNLYSISSL SISSSPSPLP LHSPDPVKGN SLPINYPETP HLWKNSETDF MTNQREQLNH 480
NSFAHIAPIN TKAITSPSRT ATPKLQRFPQ TISMNLNMNS NGHSSATSTI QPSCLSLSNN 540
DSLDHTDVTP TSSHNYDLDF AVGLENLGNS CYMNCIIQCI LGTHELTQIF LDDSYAKHIN 600
INSKLGSKGI LAKYFARLVH MMYKEQVDGS KKISISPIKF KLACGSVNSL FKTASQQDCQ 660
EFCQFLLDGL HEDLNQCGSN PPLKELSQEA EARREKLSLR IASSIEWERF LTTDFSVIVD 720
LFQGQYASRL KCKVCSHTST TYQPFTVLSI PIPKKNSRNN ITIEDCFREF TKCENLEVDE 780
QWLCPHCEKR QPSTKQLTIT RLPRNLIVHL KRFDNLLNKN NDFVIYPFLL DLTPFWANDF 840
DGVFPPGVND DELPIRGQIP PFKYELYGVA CHFGTLYGGH YTAYVKKGLK KGWLYFDDTK 900
YKPVKNKADA INSNAYVLFY HRVYGV 926 |
Gene Ontology | GO:0005768; C:endosome; IDA:SGD. GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell. GO:0005739; C:mitochondrion; IDA:SGD. GO:0000502; C:proteasome complex; IPI:SGD. GO:0004175; F:endopeptidase activity; IGI:SGD. GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro. GO:0004843; F:ubiquitin-specific protease activity; IDA:SGD. GO:0006897; P:endocytosis; IMP:SGD. GO:0010995; P:free ubiquitin chain depolymerization; IDA:SGD. GO:0016579; P:protein deubiquitination; TAS:SGD. GO:0006275; P:regulation of DNA replication; IMP:SGD. GO:0010992; P:ubiquitin homeostasis; IMP:SGD. GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |