CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002536
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-1-antitrypsin 1-1 
Protein Synonyms/Alias
 AAT; Alpha-1 protease inhibitor 1; Alpha-1-antiproteinase; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; Serpin A1a 
Gene Name
 Serpina1a 
Gene Synonyms/Alias
 Dom1; Spi1-1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
147NDLKLVEKFLEEAKNubiquitination[1]
173AESEEAKKVINDFVEubiquitination[1]
181VINDFVEKGTQGKIAubiquitination[1]
287HLEQTLSKELISKFLubiquitination[1]
292LSKELISKFLLNRRRacetylation[2, 3, 4, 5, 6]
292LSKELISKFLLNRRRubiquitination[1]
318ISGEYNLKTLMSPLGubiquitination[1]
347TEENAPLKLSQAVHKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [5] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654
Functional Description
 Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. 
Sequence Annotation
 REGION 368 387 RCL.
 CARBOHYD 64 64 N-linked (GlcNAc...).
 CARBOHYD 101 101 N-linked (GlcNAc...).
 CARBOHYD 265 265 N-linked (GlcNAc...) (Potential).  
Keyword
 Acute phase; Complete proteome; Glycoprotein; Protease inhibitor; Reference proteome; Secreted; Serine protease inhibitor; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 413 AA 
Protein Sequence
MTPSISWGLL LLAGLCCLVP SFLAEDVQET DTSQKDQSPA SHEIATNLGD FAISLYRELV 60
HQSNTSNIFF SPVSIATAFA MLSLGSKGDT HTQILEGLQF NLTQTSEADI HKSFQHLLQT 120
LNRPDSELQL STGNGLFVNN DLKLVEKFLE EAKNHYQAEV FSVNFAESEE AKKVINDFVE 180
KGTQGKIAEA VKKLDQDTVF ALANYILFKG KWKKPFDPEN TEEAEFHVDE STTVKVPMMT 240
LSGMLHVHHC STLSSWVLLM DYAGNATAVF LLPDDGKMQH LEQTLSKELI SKFLLNRRRR 300
LAQIHFPRLS ISGEYNLKTL MSPLGITRIF NNGADLSGIT EENAPLKLSQ AVHKAVLTID 360
ETGTEAAAVT VLQMVPMSMP PILRFDHPFL FIIFEEHTQS PIFLGKVVDP THK 413 
Gene Ontology
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
 GO:0006953; P:acute-phase response; IEA:UniProtKB-KW. 
Interpro
 IPR023795; Protease_inhib_I4_serpin_CS.
 IPR023796; Serpin_dom.
 IPR000215; Serpin_fam. 
Pfam
 PF00079; Serpin 
SMART
 SM00093; SERPIN 
PROSITE
 PS00284; SERPIN 
PRINTS