CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000114
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Podocalyxin 
Protein Synonyms/Alias
 GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1 
Gene Name
 PODXL 
Gene Synonyms/Alias
 PCLP; PCLP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
493TSSEMQEKKVVSLNGubiquitination[1]
494SSEMQEKKVVSLNGEubiquitination[1]
515VPLDNLTKDDLDEEEubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. 
Sequence Annotation
 MOD_RES 529 529 Phosphoserine.
 MOD_RES 537 537 Phosphoserine.
 MOD_RES 556 556 Phosphothreonine.
 CARBOHYD 33 33 N-linked (GlcNAc...) (Potential).
 CARBOHYD 43 43 N-linked (GlcNAc...) (Potential).
 CARBOHYD 104 104 N-linked (GlcNAc...) (Potential).
 CARBOHYD 144 144 N-linked (GlcNAc...) (Potential).
 CARBOHYD 360 360 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Cell adhesion; Cell membrane; Cell projection; Complete proteome; Direct protein sequencing; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 558 AA 
Protein Sequence
MRCALALSAL LLLLSTPPLL PSSPSPSPSP SQNATQTTTD SSNKTAPTPA SSVTIMATDT 60
AQQSTVPTSK ANEILASVKA TTLGVSSDSP GTTTLAQQVS GPVNTTVARG GGSGNPTTTI 120
ESPKSTKSAD TTTVATSTAT AKPNTTSSQN GAEDTTNSGG KSSHSVTTDL TSTKAEHLTT 180
PHPTSPLSPR QPTSTHPVAT PTSSGHDHLM KISSSSSTVA IPGYTFTSPG MTTTLPSSVI 240
SQRTQQTSSQ MPASSTAPSS QETVQPTSPA TALRTPTLPE TMSSSPTAAS TTHRYPKTPS 300
PTVAHESNWA KCEDLETQTQ SEKQLVLNLT GNTLCAGGAS DEKLISLICR AVKATFNPAQ 360
DKCGIRLASV PGSQTVVVKE ITIHTKLPAK DVYERLKDKW DELKEAGVSD MKLGDQGPPE 420
EAEDRFSMPL IITIVCMASF LLLVAALYGC CHQRLSQRKD QQRLTEELQT VENGYHDNPT 480
LEVMETSSEM QEKKVVSLNG ELGDSWIVPL DNLTKDDLDE EEDTHL 526 
Gene Ontology
 GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0030175; C:filopodium; IDA:UniProtKB.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
 GO:0031528; C:microvillus membrane; ISS:UniProtKB.
 GO:0001726; C:ruffle; IDA:UniProtKB.
 GO:0036057; C:slit diaphragm; ISS:UniProtKB.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0016477; P:cell migration; ISS:UniProtKB.
 GO:0072175; P:epithelial tube formation; ISS:UniProtKB.
 GO:0072015; P:glomerular visceral epithelial cell development; ISS:UniProtKB.
 GO:0050900; P:leukocyte migration; IEA:Compara.
 GO:0022408; P:negative regulation of cell-cell adhesion; ISS:UniProtKB.
 GO:0030335; P:positive regulation of cell migration; IDA:UniProtKB.
 GO:0033634; P:positive regulation of cell-cell adhesion mediated by integrin; IDA:UniProtKB.
 GO:0032534; P:regulation of microvillus assembly; ISS:UniProtKB. 
Interpro
 IPR013836; CD34/Podocalyxin.
 IPR017403; Podocalyxin-like_p1. 
Pfam
 PF06365; CD34_antigen 
SMART
  
PROSITE
  
PRINTS