UniProt Accession

 IF4A1_MOUSE; P60843; P04765; Q61516 

Genbank Protein ID

 X03039; X03039; X03040; X03040; L36611; M22873; L36608; L36609; L36610; AB011595; AK077429; BC049915 

Genbank Nucleotide ID

 CAA26842.1; CAA26843.1; CAA26845.1; CAA26846.1; AAA50407.1; AAA50407.1; AAA50407.1; AAA50407.1; AAA50407.1; BAA25075.1; BAC36796.1; AAH49915.1 

Protein Name

 Eukaryotic initiation factor 4A-I 

Protein Synonyms/Alias

 eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1 

Gene Name

 Eif4a1 

Gene Synonyms/Alias

 Ddx2a; Eif4a 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
54	IYAYGFEKPSAIQQR	ubiquitination	[1]
68	RAILPCIKGYDVIAQ	acetylation	[2]
68	RAILPCIKGYDVIAQ	ubiquitination	[1]
118	ELAQQIQKVVMALGD	acetylation	[2]
146	NVRAEVQKLQMEAPH	acetylation	[2, 3, 4]
146	NVRAEVQKLQMEAPH	ubiquitination	[1]
174	NRRYLSPKYIKMFVL	ubiquitination	[1]
177	YLSPKYIKMFVLDEA	acetylation	[2]
193	EMLSRGFKDQIYDIF	acetylation	[2, 4, 5]
193	EMLSRGFKDQIYDIF	ubiquitination	[1]
225	SDVLEVTKKFMRDPI	acetylation	[3]
225	SDVLEVTKKFMRDPI	ubiquitination	[1]
238	PIRILVKKEELTLEG	acetylation	[2, 4]
238	PIRILVKKEELTLEG	ubiquitination	[1]
291	KVDWLTEKMHARDFT	ubiquitination	[1]
309	MHGDMDQKERDVIMR	acetylation	[3]
309	MHGDMDQKERDVIMR	ubiquitination	[1]
369	RGGRFGRKGVAINMV	ubiquitination	[1]
381	NMVTEEDKRTLRDIE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. 

Sequence Annotation

 DOMAIN 63 234 Helicase ATP-binding.
 DOMAIN 245 406 Helicase C-terminal.
 NP_BIND 76 83 ATP (By similarity).
 MOTIF 32 60 Q motif.
 MOTIF 182 185 DEAD box.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 4 4 Phosphoserine (By similarity).
 MOD_RES 118 118 N6-acetyllysine (By similarity).
 MOD_RES 174 174 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Helicase; Hydrolase; Initiation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 406 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS