CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015386
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 24 
Protein Synonyms/Alias
 Kainate receptor-interacting protein for GluR6; KRIP6; Protein DRE1 
Gene Name
 KLHL24 
Gene Synonyms/Alias
 DRE1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
25DSPATKRKVFEMDPKubiquitination[1]
181FADTHSLKTLFTKCKubiquitination[1, 2, 3, 4, 5, 6]
186SLKTLFTKCKNFALQubiquitination[1]
347GEWKSLAKLPEFTKSubiquitination[1]
353AKLPEFTKSEYAVCAubiquitination[1]
441WTEVAPLKEAVSSPAubiquitination[1, 3]
471DDNTCSDKVQSYDPEubiquitination[1, 3]
492RAAIPIAKRCITAVSubiquitination[1]
545GMSVCNGKIYILGGRubiquitination[1]
596TIHRYNEKCFKL***ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Specifically reduces kainate receptor-mediated currents in hippocampal neurons, most probably by modulating channel properties (By similarity). 
Sequence Annotation
 DOMAIN 66 133 BTB.
 DOMAIN 168 270 BACK.
 REPEAT 314 363 Kelch 1.
 REPEAT 365 407 Kelch 2.
 REPEAT 408 454 Kelch 3.
 REPEAT 456 502 Kelch 4.
 REPEAT 504 544 Kelch 5.
 REPEAT 546 592 Kelch 6.  
Keyword
 Alternative splicing; Cell projection; Complete proteome; Cytoplasm; Kelch repeat; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 600 AA 
Protein Sequence
MVLILGRRLN REDLGVRDSP ATKRKVFEMD PKSLTGHEFF DFSSGSSHAE NILQIFNEFR 60
DSRLFTDVII CVEGKEFPCH RAVLSACSSY FRAMFCNDHR ESREMLVEIN GILAEAMECF 120
LQYVYTGKVK ITTENVQYLF ETSSLFQISV LRDACAKFLE EQLDPCNCLG IQRFADTHSL 180
KTLFTKCKNF ALQTFEDVSQ HEEFLELDKD ELIDYICSDE LVIGKEEMVF EAVMRWVYRA 240
VDLRRPLLHE LLTHVRLPLL HPNYFVQTVE VDQLIQNSPE CYQLLHEARR YHILGNEMMS 300
PRTRPRRSTG YSEVIVVVGG CERVGGFNLP YTECYDPVTG EWKSLAKLPE FTKSEYAVCA 360
LRNDILVSGG RINSRDVWIY NSQLNIWIRV ASLNKGRWRH KMAVLLGKVY VVGGYDGQNR 420
LSSVECYDSF SNRWTEVAPL KEAVSSPAVT SCVGKLFVIG GGPDDNTCSD KVQSYDPETN 480
SWLLRAAIPI AKRCITAVSL NNLIYVAGGL TKAIYCYDPV EDYWMHVQNT FSRQENCGMS 540
VCNGKIYILG GRRENGEATD TILCYDPATS IITGVAAMPR PVSYHGCVTI HRYNEKCFKL 600 
Gene Ontology
 GO:0030424; C:axon; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015916; Gal_Oxidase_b-propeller.
 IPR017096; Kelch-like_gigaxonin.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS