| Tag | Content |
|---|
| CPLM ID | CPLM-035894 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Isoleucine--tRNA ligase |
Protein Synonyms/Alias | Isoleucyl-tRNA synthetase |
Gene Name | ileS |
Gene Synonyms/Alias | EAM_0655 |
Created Date | July 27, 2013 |
Organism | Erwinia amylovora (strain ATCC 49946 / CCPPB 0273 / Ea273 / 27-3) |
NCBI Taxa ID | 716540 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 851 | ADARLADKLSGLGEE | acetylation | [1] |
|
Reference | [1] Differential lysine acetylation profiles of Erwinia amylovora strains revealed by proteomics. Wu X, Vellaichamy A, Wang D, Zamdborg L, Kelleher NL, Huber SC, Zhao Y. J Proteomics. 2013 Feb 21;79:60-71. [ PMID: 23234799] |
Functional Description | Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity). |
Sequence Annotation | MOTIF 58 68 "HIGH" region (By similarity).
MOTIF 602 606 "KMSKS" region (By similarity).
METAL 901 901 Zinc (By similarity).
METAL 904 904 Zinc (By similarity).
METAL 921 921 Zinc (By similarity).
METAL 924 924 Zinc (By similarity).
BINDING 561 561 Aminoacyl-adenylate (By similarity).
BINDING 605 605 ATP (By similarity). |
Keyword | Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 938 AA |
Protein Sequence | MSDYKSTLNL PETGFPMRGD LAKREPGMLQ RWYDDKLYSV IREAKKGKKT FILHDGPPYA 60
NGSIHIGHSV NKILKDIIVK SKGLAGYDSP YVPGWDCHGL PIEHKVEQTI GKPGEKVSAA 120
EFRAACREYA ARQVEGQKAD FIRLGVLGDW DRPYLTMDFK TEANIIRALG KIIGNGHLHK 180
GAKPVHWCMD CRSALAEAEV EYYDKTSPSI DVMFNAVDKD AVQAKFGAAS VDGPISLVIW 240
TTTPWTMPAN RAIALHPEFE YQLVQIEGRA LILAKDMVDS VMKRVGVPQW TVLGNVQGAA 300
LELMGFRHPF LAQVSPVVPG EHVTLEAGTG AVHTAPGHGP DDYVIGQKYG IETANPVGPD 360
GSFLPGTYPT LDGVNVFKAN DIIVELLREK GALLHVEKLH HSYPHCWRHK TPIIFRATPQ 420
WFISMDQKGL RAQSLKEIKG VQWIPDWGQA RIESMVASRP DWCISRQRTW GVPMALFVHK 480
DTEQLHPDSL ELMEKVAVRV EQDGIQAWWD LDARELMGAD ADNYVKVPDT LDVWFDSGST 540
SYSVVEARPE FGGNAPDMYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG 600
RKMSKSLGNT VSPQDVMNKL GADILRLWVA STDYSGEIAV SDEILKRSAD SYRRIRNTAR 660
FLLANLAGFN PETDKVKPEE MVVVDRWAVG RALAAQNDII ASYEAYDFHE VVQRLMQFCS 720
VEMGSFYLDI IKDRQYTAKA DGLARRSCQT ALWYIAEALV RWMAPIMSFT ADEIWGYLPG 780
KRAQYVFTEE WFDGLFSLAD NQPMNDAYWA ELLKVRGEVN KVIEQARADK HVGGSLEASV 840
TLYADARLAD KLSGLGEELR FVLLTSGAEV ADYAQAPDNA QQSETVKGLK IALSKAEGEK 900
CPRCWHYTGD IGQNAGQAEM CGRCVTNVAG SGEERKFA 938 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro. GO:0005524; F:ATP binding; IEA:HAMAP. GO:0004822; F:isoleucine-tRNA ligase activity; IEA:HAMAP. GO:0008270; F:zinc ion binding; IEA:HAMAP. GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:HAMAP. GO:0006450; P:regulation of translational fidelity; IEA:GOC. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |