CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013675
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 44 
Protein Synonyms/Alias
 Rabphilin-11 
Gene Name
 WDR44 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
440GKSVKRAKHLAEEYGubiquitination[1]
518MGAVWTMKFSHCGRLubiquitination[2, 3]
671LSGSLDGKLRLWNIPubiquitination[4]
756EPLPGENKILVTSNDubiquitination[1, 2, 3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 Downstream effector for RAB11. May be involved in vesicle recycling (By similarity). 
Sequence Annotation
 REPEAT 509 548 WD 1.
 REPEAT 605 643 WD 2.
 REPEAT 645 685 WD 3.
 REPEAT 690 729 WD 4.
 REPEAT 740 779 WD 5.
 REPEAT 784 823 WD 6.
 REPEAT 876 913 WD 7.
 REGION 2 170 Binding activity.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 3 3 Phosphoserine.
 MOD_RES 11 11 Phosphotyrosine.
 MOD_RES 27 27 Phosphoserine.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 219 219 Phosphothreonine.
 MOD_RES 262 262 Phosphoserine.
 MOD_RES 271 271 Phosphothreonine.
 MOD_RES 342 342 Phosphoserine.
 MOD_RES 344 344 Phosphoserine (By similarity).
 MOD_RES 349 349 Phosphothreonine (By similarity).
 MOD_RES 403 403 Phosphoserine.
 MOD_RES 470 470 Phosphoserine.
 MOD_RES 471 471 Phosphoserine.
 MOD_RES 472 472 Phosphoserine.
 MOD_RES 561 561 Phosphoserine.
 MOD_RES 565 565 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Endosome; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 913 AA 
Protein Sequence
MASESDTEEF YDAPEDVHLG GGYPVGSPGK VGLSTFKETE NTAYKVGNES PVQELKQDVS 60
KKIIESIIEE SQKVLQLEDD SLDSKGKELS DQATASPIVA RTDLSNIPGL LAIDQVLPEE 120
SQKAESQNTF EETELELKKC FPSDETCEKP VDETTKLTQT SSTEQLNVLE TETEVLNKEA 180
VEVKGGGDVL EPVSSDSLST KDFAAVEEVA PAKPPRHLTP EPDIVASTKK PVPARPPPPT 240
NFPPPRPPPP SRPAPPPRKR KSELEFETLK TPDIDVPKEN ITSDSLLTAS MASESTVKDS 300
QPSLDLASAT SGDKIVTAQE NGKAPDGQTV AGEVMGPQRP RSNSGRELTD EEILASVMIK 360
NLDTGEEIPL SLAEEKLPTG INPLTLHIMR RTKEYVSNDA AQSDDEEKLQ SQPTDTDGGR 420
LKQKTTQLKK FLGKSVKRAK HLAEEYGERA INKVKSVRDE VFHTDQDDPS SSDDEGMPYT 480
RPVKFKAAHG FKGPYDFDQI KVVQDLSGEH MGAVWTMKFS HCGRLLASAG QDNVVRIWAL 540
KNAFDYFNNM RMKYNTEGRV SPSPSQESLS SSKSDTDTGV CSGTDEDPDD KNAPFRQRPF 600
CKYKGHTADL LDLSWSKNYF LLSSSMDKTV RLWHISRREC LCCFQHIDFV TAIAFHPRDD 660
RYFLSGSLDG KLRLWNIPDK KVALWNEVDG QTKLITAANF CQNGKYAVIG TYDGRCIFYD 720
TEHLKYHTQI HVRSTRGRNK VGRKITGIEP LPGENKILVT SNDSRIRLYD LRDLSLSMKY 780
KGYVNSSSQI KASFSHDFTY LVSGSEDKYV YIWSTYHDLS KFTSVRRDRN DFWEGIKAHN 840
AVVTSAIFAP NPSLMLSLDV QSEKSEGNEK SEDAEVLDAT PSGIMKTDNT EVLLSADFTG 900
AIKVFVNKRK NVS 913 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.