UniProt Accession

 SUB2_YEAST; Q07478; D6VRR5 

Genbank Protein ID

 Z74132; AY692907; BK006938 

Genbank Nucleotide ID

 CAA98650.1; AAT92926.1; DAA11775.1 

Protein Name

 ATP-dependent RNA helicase SUB2 

Protein Synonyms/Alias

 Suppressor of BRR1 protein 2 

Gene Name

 SUB2 

Gene Synonyms/Alias

 YDL084W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
52	NNTAAGDKKGSYVGI	ubiquitination	[1, 2]
70	GFKDFLLKPELSRAI	acetylation	[3]
155	NEYLRFSKYMPDVKT	acetylation	[3]
173	YGGTPISKDAELLKN	acetylation	[3]
202	LKALVREKYIDLSHV	acetylation	[3]
324	TRANELTKLLNASNF	acetylation	[3]
341	ITVHGHMKQEERIAR	acetylation	[3]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 ATP-binding RNA helicase component of the TREX complex involved in transcription elongation and required for the export of mRNA out of the nucleus. SUB2 plays also a role in pre-mRNA splicing and spliceosome assembly. May be involved in rDNA and telomeric silencing, and maintenance of genome integrity. 

Sequence Annotation

 DOMAIN 93 268 Helicase ATP-binding.
 DOMAIN 280 441 Helicase C-terminal.
 NP_BIND 106 113 ATP.
 MOTIF 62 90 Q motif.
 MOTIF 215 218 DECD box.
 MOD_RES 13 13 Phosphoserine.
 MOD_RES 37 37 Phosphoserine.
 MOD_RES 169 169 Phosphothreonine.  

Keyword

 ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing; mRNA splicing; mRNA transport; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 446 AA 

Protein Sequence

Gene Ontology

 GO:0000781; C:chromosome, telomeric region; IPI:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005681; C:spliceosomal complex; TAS:SGD.
 GO:0000346; C:transcription export complex; IPI:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; ISA:SGD.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006348; P:chromatin silencing at telomere; IMP:SGD.
 GO:0031124; P:mRNA 3'-end processing; IMP:SGD.
 GO:0006406; P:mRNA export from nucleus; IDA:SGD.
 GO:0000398; P:mRNA splicing, via spliceosome; IMP:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; IMP:SGD. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS