CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012668
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-internexin 
Protein Synonyms/Alias
 Alpha-Inx; 66 kDa neurofilament protein; NF-66; Neurofilament-66; Neurofilament 5 
Gene Name
 INA 
Gene Synonyms/Alias
 NEF5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
95KIIRTNEKEQLQGLNubiquitination[1]
290AEEWYKSKFANLNEQacetylation[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. 
Sequence Annotation
 REGION 1 87 Head.
 REGION 88 408 Rod.
 REGION 88 129 Coil 1A.
 REGION 130 142 Linker 1.
 REGION 143 238 Coil 1B.
 REGION 239 262 Linker 2.
 REGION 263 408 Coil 2.
 REGION 409 499 Tail.
 MOD_RES 72 72 Phosphoserine (By similarity).
 MOD_RES 290 290 N6-acetyllysine.
 MOD_RES 335 335 Phosphoserine (By similarity).
 MOD_RES 496 496 Phosphoserine.  
Keyword
 Acetylation; Coiled coil; Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; Glycoprotein; Intermediate filament; Neurogenesis; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 499 AA 
Protein Sequence
MSFGSEHYLC SSSSYRKVFG DGSRLSARLS GAGGAGGFRS QSLSRSNVAS SAACSSASSL 60
GLGLAYRRPP ASDGLDLSQA AARTNEYKII RTNEKEQLQG LNDRFAVFIE KVHQLETQNR 120
ALEAELAALR QRHAEPSRVG ELFQRELRDL RAQLEEASSA RSQALLERDG LAEEVQRLRA 180
RCEEESRGRE GAERALKAQQ RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA 240
TLQASSQAAA EVDVTVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR 300
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE VAGYQDSIGQ 360
LENDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL EGEETRFSTS GLSISGLNPL 420
PNPSYLLPPR ILSATTSKVS STGLSLKKEE EEEEASKVAS KKTSQIGESF EEILEETVIS 480
TKKTEKSNIE ETTISSQKI 499 
Gene Ontology
 GO:0005883; C:neurofilament; TAS:ProtInc.
 GO:0005200; F:structural constituent of cytoskeleton; TAS:ProtInc.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0060052; P:neurofilament cytoskeleton organization; IEA:Compara. 
Interpro
 IPR016044; F.
 IPR001664; IF.
 IPR006821; Intermed_filament_DNA-bd.
 IPR018039; Intermediate_filament_CS. 
Pfam
 PF00038; Filament
 PF04732; Filament_head 
SMART
  
PROSITE
 PS00226; IF 
PRINTS