CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011523
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sterol esterase 1 
Protein Synonyms/Alias
 Steryl ester hydrolase 1 
Gene Name
 YEH1 
Gene Synonyms/Alias
 YLL012W; L1329 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
56IEVDDESKRNVHHDHubiquitination[1]
82YLDLEHDKKNGIVYDubiquitination[1]
137TEGVTQGKFNNSSNEubiquitination[1]
273RPEWNEAKVPTLASRubiquitination[1]
303LIDTVLAKTQFEKLTubiquitination[1]
463SFKFGSHKMFPDNVKubiquitination[1]
535DVIERIGKPILQNLNubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Mediates the hydrolysis of steryl esters, thereby playing a central role in lipid metabolism. Under heme-deficient conditions, it constitutes the major steryl ester hydrolase, suggesting that it plays a central role in mobilization of steryl esters under anaerobic conditions. 
Sequence Annotation
 ACT_SITE 315 315 Nucleophile (By similarity).
 ACT_SITE 489 489 Charge relay system (By similarity).
 ACT_SITE 520 520 Charge relay system (By similarity).  
Keyword
 Complete proteome; Hydrolase; Lipid degradation; Lipid droplet; Lipid metabolism; Membrane; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 573 AA 
Protein Sequence
MGVSAVLKRA RNLLATFIVC CFMAVVLVLA LAHHFINEHR DTRSSSTQIE VDDESKRNVH 60
HDHVLTRTNA YATPYLDLEH DKKNGIVYDH TRTVVRKKNH EVGSSSLHKN LFHKFLTKLI 120
FRFIEKEKVT EGVTQGKFNN SSNEIANHEP VFEKIPVQCD NPLQNLILSE DLTLVADLNY 180
YFNQYNIQIE EFRLETEDGF VIDLWHLIPK YRTTDSDKKK RPPILMLHGL LQSSGSFASN 240
GRKSLAYFLY QSGYDIWLGN NRCGFRPEWN EAKVPTLASR WDWDLREMVK YDLTLLIDTV 300
LAKTQFEKLT LISHSQGTTQ GFMGLVNEDK FFPPGSGSKE SFFTSKIANY IALAPAVYPG 360
PLLNEKLFVK LMTKEIENPW FFGETSFFEI MMIVRNLCVG ESLFSFVCYT IFNYLFDWND 420
TLWDTALRDR HFLFSPVHVS VKLMQWWLSP DPNKVSFKFG SHKMFPDNVK WFSDASKAPN 480
IYLFVPKQDR LVDGERLINH FVNVESNVNY KIWYIDEYAH IDVLWAHDVI ERIGKPILQN 540
LNNYYSKKPS SAFESDCSDT EVETELEMVA EKA 573 
Gene Ontology
 GO:0016021; C:integral to membrane; IDA:SGD.
 GO:0005811; C:lipid particle; IDA:SGD.
 GO:0004771; F:sterol esterase activity; IDA:SGD.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0016125; P:sterol metabolic process; IMP:SGD. 
Interpro
 IPR006693; AB_hydrolase_lipase. 
Pfam
 PF04083; Abhydro_lipase 
SMART
  
PROSITE
 PS00120; LIPASE_SER 
PRINTS