CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021047
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transient receptor potential cation channel subfamily V member 4 
Protein Synonyms/Alias
 TrpV4; Osm-9-like TRP channel 4; OTRPC4; Transient receptor potential protein 12; TRP12; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; Vanilloid receptor-related osmotically-activated channel; VR-OAC 
Gene Name
 Trpv4 
Gene Synonyms/Alias
 Trp12; Vrl2; Vroac 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
177LSFLLTHKKRLTDEEubiquitination[1]
192FREPSTGKTCLPKALubiquitination[1]
197TGKTCLPKALLNLSNubiquitination[1]
344ENTKFVTKMYDLLLLubiquitination[1]
352MYDLLLLKCSRLFPDubiquitination[1]
766GEMVTVGKSSDGTPDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13- didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. 
Sequence Annotation
 REPEAT 237 266 ANK 1.
 REPEAT 284 313 ANK 2.
 REPEAT 369 398 ANK 3.
 REGION 812 831 Interaction with calmodulin (By
 BINDING 192 192 ATP (By similarity).
 BINDING 201 201 ATP (By similarity).
 BINDING 239 239 ATP (By similarity).
 BINDING 248 248 ATP (By similarity).
 MOD_RES 253 253 Phosphotyrosine; by LYN (Probable).
 MOD_RES 824 824 Phosphoserine; by PKC and PKA (By  
Keyword
 ANK repeat; ATP-binding; Calcium; Calcium channel; Calcium transport; Calmodulin-binding; Cell junction; Cell membrane; Complete proteome; Ion channel; Ion transport; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 871 AA 
Protein Sequence
MADPGDGPRA APGEVAEPPG DESGTSGGEA FPLSSLANLF EGEEGSSSLS PVDASRPAGP 60
GDGRPNLRMK FQGAFRKGVP NPIDLLESTL YESSVVPGPK KAPMDSLFDY GTYRHHPSDN 120
KRWRRKVVEK QPQSPKAPAP QPPPILKVFN RPILFDIVSR GSTADLDGLL SFLLTHKKRL 180
TDEEFREPST GKTCLPKALL NLSNGRNDTI PVLLDIAERT GNMREFINSP FRDIYYRGQT 240
SLHIAIERRC KHYVELLVAQ GADVHAQARG RFFQPKDEGG YFYFGELPLS LAACTNQPHI 300
VNYLTENPHK KADMRRQDSR GNTVLHALVA IADNTRENTK FVTKMYDLLL LKCSRLFPDS 360
NLETVLNNDG LSPLMMAAKT GKIGVFQHII RREVTDEDTR HLSRKFKDWA YGPVYSSLYD 420
LSSLDTCGEE VSVLEILVYN SKIENRHEML AVEPINELLR DKWRKFGAVS FYINVVSYLC 480
AMVIFTLTAY YQPLEGTPPY PYRTTVDYLR LAGEVITLFT GVLFFFTSIK DLFTKKCPGV 540
NSLFVDGSFQ LLYFIYSVLV VVSAALYLAG IEAYLAVMVF ALVLGWMNAL YFTRGLKLTG 600
TYSIMIQKIL FKDLFRFLLV YLLFMIGYAS ALVTLLNPCT NMKVCDEDQS NCTVPTYPAC 660
RDSETFSAFL LDLFKLTIGM GDLEMLSSAK YPVVFILLLV TYIILTFVLL LNMLIALMGE 720
TVGQVSKESK HIWKLQWATT ILDIERSFPV FLRKAFRSGE MVTVGKSSDG TPDRRWCFRV 780
DEVNWSHWNQ NLGIINEDPG KSEIYQYYGF SHTVGRLRRD RWSSVVPRVV ELNKNSSADE 840
VVVPLDNLGN PNCDGHQQGY APKWRTDDAP L 871 
Gene Ontology
 GO:0005912; C:adherens junction; IDA:UniProtKB.
 GO:0005929; C:cilium; IDA:MGI.
 GO:0030864; C:cortical actin cytoskeleton; IEA:Compara.
 GO:0005881; C:cytoplasmic microtubule; IEA:Compara.
 GO:0030175; C:filopodium; IEA:Compara.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0030426; C:growth cone; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0032587; C:ruffle membrane; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005262; F:calcium channel activity; IMP:UniProtKB.
 GO:0005516; F:calmodulin binding; ISS:UniProtKB.
 GO:0005034; F:osmosensor activity; IDA:MGI.
 GO:0031532; P:actin cytoskeleton reorganization; IEA:Compara.
 GO:0007015; P:actin filament organization; IEA:Compara.
 GO:0070509; P:calcium ion import; IDA:BHF-UCL.
 GO:0007043; P:cell-cell junction assembly; IMP:UniProtKB.
 GO:0006874; P:cellular calcium ion homeostasis; IMP:UniProtKB.
 GO:0071476; P:cellular hypotonic response; IDA:BHF-UCL.
 GO:0034605; P:cellular response to heat; IMP:UniProtKB.
 GO:0043622; P:cortical microtubule organization; IEA:Compara.
 GO:0007204; P:elevation of cytosolic calcium ion concentration; IEA:Compara.
 GO:0042538; P:hyperosmotic salinity response; IMP:MGI.
 GO:0046785; P:microtubule polymerization; IEA:Compara.
 GO:0010977; P:negative regulation of neuron projection development; IEA:Compara.
 GO:0007231; P:osmosensory signaling pathway; IDA:BHF-UCL.
 GO:0031117; P:positive regulation of microtubule depolymerization; IEA:Compara.
 GO:0047484; P:regulation of response to osmotic stress; IMP:MGI.
 GO:0030103; P:vasopressin secretion; IMP:MGI. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR005821; Ion_trans_dom.
 IPR004729; TRP_channel.
 IPR008347; TRPV1-4_channel.
 IPR008348; TRPV4_channel.
 IPR024862; TRPV_channel. 
Pfam
 PF12796; Ank_2
 PF00520; Ion_trans 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS
 PR01768; TRPVRECEPTOR.
 PR01769; VRL2RECEPTOR.