CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017570
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 23 
Protein Synonyms/Alias
  
Gene Name
 KLHL23 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MALKGQEDYIYubiquitination[1]
251SCLLTENKIRSLIYNubiquitination[2, 3]
265NALNPMHKEISQRSTubiquitination[3, 4, 5]
379YALGGYRKGAPAEEAubiquitination[3, 5]
393AEFYDPLKEKWIPIAubiquitination[3, 5]
395FYDPLKEKWIPIANMubiquitination[3]
529TYLQSIEKYDPDLNKubiquitination[3]
536KYDPDLNKWEIVGNLubiquitination[3]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
 DOMAIN 36 104 BTB.
 DOMAIN 139 240 BACK.
 REPEAT 274 320 Kelch 1.
 REPEAT 321 369 Kelch 2.
 REPEAT 370 416 Kelch 3.
 REPEAT 418 466 Kelch 4.
 REPEAT 467 508 Kelch 5.
 REPEAT 510 557 Kelch 6.  
Keyword
 Complete proteome; Kelch repeat; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 558 AA 
Protein Sequence
MALKGQEDYI YLFKDSTHPV DFLDAFRTFY LDGLFTDITL QCPSGIIFHC HRAVLAACSN 60
YFKAMFTADM KEKFKNKIKL SGIHHDILEG LVNYAYTSQI EITKRNVQSL LEAADLLQFL 120
SVKKACERFL VRHLDIDNCI GMHSFAEFHV CPELEKESRR ILCSKFKEVW QQEEFLEISL 180
EKFLFILSRK NLSVWKEEAI IEPVIKWTAH DVENRIECLY NLLSYINIDI DPVYLKTALG 240
LQRSCLLTEN KIRSLIYNAL NPMHKEISQR STATMYIIGG YYWHPLSEVH IWDPLTNVWI 300
QGAEIPDYTR ESYGVTCLGP NIYVTGGYRT DNIEALDTVW IYNSESDEWT EGLPMLNARY 360
YHCAVTLGGC VYALGGYRKG APAEEAEFYD PLKEKWIPIA NMIKGVGNAT ACVLHDVIYV 420
IGGHCGYRGS CTYDKVQSYN SDINEWSLIT SSPHPEYGLC SVPFENKLYL VGGQTTITEC 480
YDPEQNEWRE IAPMMERRME CGAVIMNGCI YVTGGYSYSK GTYLQSIEKY DPDLNKWEIV 540
GNLPSAMRSH GCVCVYNV 558 
Gene Ontology
  
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015916; Gal_Oxidase_b-propeller.
 IPR017096; Kelch-like_gigaxonin.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS