CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010974
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar transporter chaperone 3 
Protein Synonyms/Alias
 Phosphate metabolism protein 2 
Gene Name
 VTC3 
Gene Synonyms/Alias
 PHM2; YPL019C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
6**MLFGIKLANDVYPubiquitination[1]
30ERLKKLLKESVIHDGacetylation[2]
264ARLNIGSKSNSLSSDubiquitination[3]
282NQDVEIGKSKSVIFPubiquitination[3]
333WIGKLLDKPDIFLEKacetylation[2]
364IRLQMKAKFINNFIFacetylation[2]
486LRAGEYSKFPYSVMEacetylation[2]
581KQKSIHDKLDNMRRLacetylation[2]
654FLKILAGKNISENGNubiquitination[3]
Reference
 [1] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for LMA1 release prior to membrane fusion. 
Sequence Annotation
 DOMAIN 1 145 SPX.
 MOD_RES 43 43 Phosphoserine.
 MOD_RES 45 45 Phosphoserine.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 183 183 Phosphothreonine.
 MOD_RES 195 195 Phosphoserine.
 MOD_RES 198 198 Phosphoserine.
 MOD_RES 270 270 Phosphoserine.
 MOD_RES 274 274 Phosphoserine.
 MOD_RES 589 589 Phosphoserine.
 MOD_RES 592 592 Phosphoserine.
 MOD_RES 621 621 Phosphoserine.
 MOD_RES 622 622 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; Direct protein sequencing; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 835 AA 
Protein Sequence
MLFGIKLAND VYPPWKDSYI DYERLKKLLK ESVIHDGRSS VDSWSERNES DFVEALDKEL 60
EKVYTFQISK YNAVLRKLDD LEENTKSAEK IQKINSEQFK NTLEECLDEA QRLDNFDRLN 120
FTGFIKIVKK HDKLHPNYPS VKSLLQVRLK ELPFNNSEEY SPLLYRISYL YEFLRSNYDH 180
PNTVSKSLAS TSKLSHFSNL EDASFKSYKF WVHDDNIMEV KARILRHLPA LVYASVPNEN 240
DDFVDNLESD VRVQPEARLN IGSKSNSLSS DGNSNQDVEI GKSKSVIFPQ SYDPTITTLY 300
FDNDFFDLYN NRLLKISGAP TLRLRWIGKL LDKPDIFLEK RTFTENTETG NSSFEEIRLQ 360
MKAKFINNFI FKNDPSYKNY LINQLRERGT QKEELEKLSR DFDNIQNFIV EEKLQPVLRA 420
TYNRTAFQIP GDQSIRVTID SNIMYIREDS LDKNRPIRNP ENWHRDDIDS NIPNPLRFLR 480
AGEYSKFPYS VMEIKVINQD NSQMPNYEWI KDLTNSHLVN EVPKFSLYLQ GVASLFGEDD 540
KYVNILPFWL PDLETDIRKN PQEAYEEEKK TLQKQKSIHD KLDNMRRLSK ISVPDGKTTE 600
RQGQKDQNTR HVIADLEDHE SSDEEGTALP KKSAVKKGKK FKTNAAFLKI LAGKNISENG 660
NDPYSDDTDS ASSFQLPPGV KKPVHLLKNA GPVKVEAKVW LANERTFNRW LSVTTLLSVL 720
TFSIYNSVQK AEFPQLADLL AYVYFFLTLF CGVWAYRTYL KRLTLIKGRS GKHLDAPVGP 780
ILVAVVLIVT LVVNFSVAFK EAARRERGLV NVSSQPSLPR TLKPIQDFIF NLVGE 835 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0033254; C:vacuolar transporter chaperone complex; IPI:SGD.
 GO:0016237; P:microautophagy; IDA:SGD.
 GO:0006797; P:polyphosphate metabolic process; IMP:SGD.
 GO:0007034; P:vacuolar transport; IDA:SGD.
 GO:0042144; P:vacuole fusion, non-autophagic; IMP:SGD. 
Interpro
 IPR003807; DUF202.
 IPR004331; SPX_N.
 IPR018966; VTC_domain. 
Pfam
 PF02656; DUF202
 PF03105; SPX
 PF09359; VTC 
SMART
  
PROSITE
 PS51382; SPX 
PRINTS