UniProt Accession

 SF3A3_HUMAN; Q12874; D3DPT5; Q15460; Q5VT87 

Genbank Protein ID

 U08815; X81789; AL603790; CH471059; CH471059; BC002395; BC011523 

Genbank Nucleotide ID

 AAA19625.1; CAA57388.1; CAH69930.1; EAX07304.1; EAX07305.1; AAH02395.1; AAH11523.1 

Protein Name

 Splicing factor 3A subunit 3 

Protein Synonyms/Alias

 SF3a60; Spliceosome-associated protein 61; SAP 61 

Gene Name

 SF3A3 

Gene Synonyms/Alias

 SAP61 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
89	AEFYNRLKQIKEFHR	ubiquitination	[1]
92	YNRLKQIKEFHRKHP	acetylation	[2]
92	YNRLKQIKEFHRKHP	ubiquitination	[1]
148	DLHDCYLKYINLKAS	ubiquitination	[1]
153	YLKYINLKASEKLDY	ubiquitination	[1]
201	QDYTDRVKPLQDQNE	ubiquitination	[1, 3, 4, 5]
212	DQNELFGKIQAEFEK	ubiquitination	[1]
219	KIQAEFEKKWENGTF	acetylation	[2]
291	RLFSTKGKSLESLDT	ubiquitination	[1]
303	LDTSLFAKNPKSKGT	ubiquitination	[1]
306	SLFAKNPKSKGTKRD	ubiquitination	[1]
390	LPLGWDGKPIPYWLY	ubiquitination	[1, 3, 5]
466	WAKLKLQKASERWQP	ubiquitination	[1]
489	SSGNVVNKKTYEDLK	ubiquitination	[4]
496	KKTYEDLKRQGLL**	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. 

Sequence Annotation

 ZN_FING 406 437 Matrin-type.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 299 299 Phosphoserine.
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 367 367 Phosphoserine.
 MOD_RES 369 369 Phosphoserine.
 CARBOHYD 119 119 N-linked (GlcNAc...).  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Glycoprotein; Metal-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Spliceosome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 501 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000389; P:mRNA 3'-splice site recognition; TAS:HGNC. 

Interpro

 IPR024598; DUF3449.
 IPR021966; SF3a60_bindingd.
 IPR015880; Znf_C2H2-like.
 IPR000690; Znf_C2H2_matrin. 

Pfam

 PF11931; DUF3449
 PF12108; SF3a60_bindingd 

SMART

 SM00355; ZnF_C2H2 

PROSITE

 PS50171; ZF_MATRIN 

PRINTS