CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005344
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-adrenergic receptor kinase 1 
Protein Synonyms/Alias
 Beta-ARK-1; G-protein coupled receptor kinase 2 
Gene Name
 ADRBK1 
Gene Synonyms/Alias
 BARK; BARK1; GRK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31PAARASKKILLPEPSubiquitination[1]
45SIRSVMQKYLEDRGEubiquitination[1]
57RGEVTFEKIFSQKLGubiquitination[1, 2, 3]
62FEKIFSQKLGYLLFRubiquitination[1, 2, 3]
94EEIKKYEKLETEEERubiquitination[1]
115IFDSYIMKELLACSHubiquitination[1]
126ACSHPFSKSATEHVQubiquitination[1]
139VQGHLGKKQVPPDLFubiquitination[1]
164LRGDVFQKFIESDKFubiquitination[1]
170QKFIESDKFTRFCQWubiquitination[1, 2, 3]
215CRKADTGKMYAMKCLubiquitination[1]
220TGKMYAMKCLDKKRIubiquitination[1, 4]
230DKKRIKMKQGETLALubiquitination[1]
319FVVYRDLKPANILLDubiquitination[1]
344GLACDFSKKKPHASVubiquitination[1, 4]
345LACDFSKKKPHASVGubiquitination[1]
346ACDFSKKKPHASVGTubiquitination[1]
448GRGAQEVKESPFFRSubiquitination[1, 4, 5]
494SFDEEDTKGIKLLDSubiquitination[1, 5, 6]
497EEDTKGIKLLDSDQEubiquitination[1]
545ARKKAKNKQLGHEEDubiquitination[1, 5]
557EEDYALGKDCIMHGYubiquitination[1]
628LLKIRGGKQFILQCDubiquitination[1]
644DPELVQWKKELRDAYubiquitination[1, 4]
677SPVVELSKVPLVQRGubiquitination[1, 2, 3, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. 
Sequence Annotation
 DOMAIN 54 175 RGS.
 DOMAIN 191 453 Protein kinase.
 DOMAIN 454 521 AGC-kinase C-terminal.
 DOMAIN 558 652 PH.
 NP_BIND 197 205 ATP (By similarity).
 REGION 1 190 N-terminal.
 ACT_SITE 317 317 Proton acceptor (By similarity).
 BINDING 220 220 ATP (By similarity).
 MOD_RES 670 670 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 689 AA 
Protein Sequence
MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR GEVTFEKIFS 60
QKLGYLLFRD FCLNHLEEAR PLVEFYEEIK KYEKLETEEE RVARSREIFD SYIMKELLAC 120
SHPFSKSATE HVQGHLGKKQ VPPDLFQPYI EEICQNLRGD VFQKFIESDK FTRFCQWKNV 180
ELNIHLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 240
IMLSLVSTGD CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE 300
IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKKPHAS VGTHGYMAPE 360
VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTMA VELPDSFSPE 420
LRSLLEGLLQ RDVNRRLGCL GRGAQEVKES PFFRSLDWQM VFLQKYPPPL IPPRGEVNAA 480
DAFDIGSFDE EDTKGIKLLD SDQELYRNFP LTISERWQQE VAETVFDTIN AETDRLEARK 540
KAKNKQLGHE EDYALGKDCI MHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RGEGEAPQSL 600
LTMEEIQSVE ETQIKERKCL LLKIRGGKQF ILQCDSDPEL VQWKKELRDA YREAQQLVQR 660
VPKMKNKPRS PVVELSKVPL VQRGSANGL 689 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0031694; F:alpha-2A adrenergic receptor binding; ISS:BHF-UCL.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0047696; F:beta-adrenergic receptor kinase activity; IEA:EC.
 GO:0031755; F:Edg-2 lysophosphatidic acid receptor binding; IDA:UniProtKB.
 GO:0004703; F:G-protein coupled receptor kinase activity; ISS:BHF-UCL.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0007202; P:activation of phospholipase C activity; TAS:Reactome.
 GO:0060048; P:cardiac muscle contraction; IMP:BHF-UCL.
 GO:0002029; P:desensitization of G-protein coupled receptor protein signaling pathway; ISS:BHF-UCL.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0007213; P:G-protein coupled acetylcholine receptor signaling pathway; ISS:BHF-UCL.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0045988; P:negative regulation of striated muscle contraction; IMP:BHF-UCL.
 GO:0003108; P:negative regulation of the force of heart contraction by chemical signal; IMP:BHF-UCL.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0018105; P:peptidyl-serine phosphorylation; ISS:BHF-UCL.
 GO:0018107; P:peptidyl-threonine phosphorylation; IEA:Compara.
 GO:0033605; P:positive regulation of catecholamine secretion; ISS:BHF-UCL.
 GO:0031623; P:receptor internalization; IDA:UniProtKB.
 GO:0007217; P:tachykinin receptor signaling pathway; IDA:BHF-UCL.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR000239; GPCR_kinase.
 IPR011009; Kinase-like_dom.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR000342; Regulat_G_prot_signal.
 IPR016137; Regulat_G_prot_signal_superfam.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00169; PH
 PF00069; Pkinase
 PF00615; RGS 
SMART
 SM00233; PH
 SM00315; RGS
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS50003; PH_DOMAIN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50132; RGS 
PRINTS
 PR00717; GPCRKINASE.