CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033117
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Pyruvate dehydrogenase E1 component subunit beta, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 PDHB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50AQYDGAYKVSRGLWKacetylation[1, 2]
50AQYDGAYKVSRGLWKubiquitination[3]
166PWNSEDAKGLIKSAIubiquitination[3, 4, 5, 6]
170EDAKGLIKSAIRDNNubiquitination[5, 6]
209DFLIPIGKAKIERQGubiquitination[3]
211LIPIGKAKIERQGTHubiquitination[5]
Reference
 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 350 AA 
Protein Sequence
MAAVSGLVRR PLREVTVRDA INQGMDEELE RDEKVFLLGE EVAQYDGAYK VSRGLWKKYG 60
DKRIIDTPIS EMGFAGIAVG AAMAGLRPIC EFMTFNFSMQ AIDQVINSAA KTYYMSGGLQ 120
PVPIVFRGPN GASAGVAAQH SQCFAAWYGH CPGLKVVSPW NSEDAKGLIK SAIRDNNPVV 180
VLENELMYGV PFEFPPEAQS KDFLIPIGKA KIERQGTHIT VVSHSRPVGH CLEAAAVLSK 240
EGVECEVINM RTIRPMDMET IEASVMKTNH LVTVEGGWPQ FGVGAEICAR IMEGISKKLV 300
LEYSQAIVNI MYLNTYRIKR LKARSGQTTS TLNLSCVQFP GCSCCSCHWC 350 
Gene Ontology
 GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IEA:InterPro.
 GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IEA:InterPro. 
Interpro
 IPR027110; PDHB.
 IPR009014; Transketo_C/Pyr-ferredox_oxred.
 IPR015941; Transketolase-like_C.
 IPR005475; Transketolase-like_Pyr-bd.
 IPR005476; Transketolase_C. 
Pfam
 PF02779; Transket_pyr
 PF02780; Transketolase_C 
SMART
 SM00861; Transket_pyr 
PROSITE
  
PRINTS