UniProt Accession

 TULP3_HUMAN; O75386; B3KNB7; D3DUQ4; Q8N5B0 

Genbank Protein ID

 AF045583; AK024246; CH471116; CH471116; CH471116; BC032587 

Genbank Nucleotide ID

 AAC95431.1; BAG51279.1; EAW88876.1; EAW88877.1; EAW88878.1; AAH32587.1 

Protein Name

 Tubby-related protein 3 

Protein Synonyms/Alias

 Tubby-like protein 3 

Gene Name

 TULP3 

Gene Synonyms/Alias

 TUBL3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
245	ARKRKKSKTANYLIS	ubiquitination	[1]
277	RSNLMGTKFTVYDRG	ubiquitination	[1]
316	ETNVLGFKGPRKMSV	ubiquitination	[1, 2, 3, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Negative regulator of the Shh signaling transduction pathway: recruited to primary cilia via association with the IFT complex A (IFT-A) and is required for recruitment of G protein- coupled receptor GPR161 to cilia, a promoter of PKA-dependent basal repression machinery in Shh signaling. Binds to phosphorylated inositide (phosphoinositide) lipids. Both IFT-A- and phosphoinositide-binding properties are required to regulate ciliary G protein-coupled receptor trafficking. Not involved in ciliogenesis. 

Sequence Annotation

 REGION 23 68 Required for association with the IFT  

Keyword

 Cell membrane; Cell projection; Cilium; Complete proteome; Cytoplasm; Developmental protein; Membrane; Nucleus; Reference proteome; Secreted. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 442 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; NAS:UniProtKB.
 GO:0072372; C:primary cilium; IDA:UniProtKB.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; NAS:UniProtKB.
 GO:0007186; P:G-protein coupled receptor signaling pathway; NAS:UniProtKB.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0045879; P:negative regulation of smoothened signaling pathway; IDA:UniProtKB.
 GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; IDA:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; NAS:UniProtKB. 

Interpro

 IPR000007; Tubby_C.
 IPR025659; Tubby_C-like.
 IPR018066; Tubby_C_CS.
 IPR005398; Tubby_N. 

Pfam

 PF01167; Tub 

SMART

  

PROSITE

 PS01200; TUB_1
 PS01201; TUB_2 

PRINTS

 PR01573; SUPERTUBBY.
 PR01574; TUBBYPROTEIN.