CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004272
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar aminopeptidase 1 
Protein Synonyms/Alias
 Aminopeptidase III; Aminopeptidase yscI; Leucine aminopeptidase IV; LAPIV; Polypeptidase; Vacuolar aminopeptidase I 
Gene Name
 LAP4 
Gene Synonyms/Alias
 APE1; API; YSC1; YKL103C; YKL455 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
213SLAPHFGKPAEGPFDacetylation[1]
294GTIGGIGKHFLFAPRacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 This vacuolar enzyme catalyzes the removal of amino acids from the N-terminus of peptides and proteins. 
Sequence Annotation
 METAL 132 132 Zinc (Potential).
 METAL 210 210 Zinc (Potential).
 METAL 479 479 Zinc (Potential).
 MOD_RES 356 356 Phosphoserine.
 CARBOHYD 107 107 N-linked (GlcNAc...) (Potential).
 CARBOHYD 110 110 N-linked (GlcNAc...) (Potential).
 CARBOHYD 448 448 N-linked (GlcNAc...) (Potential).  
Keyword
 Aminopeptidase; Complete proteome; Direct protein sequencing; Glycoprotein; Hydrolase; Metal-binding; Metalloprotease; Phosphoprotein; Protease; Reference proteome; Vacuole; Zinc; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 514 AA 
Protein Sequence
MEEQREILEQ LKKTLQMLTV EPSKNNQIAN EEKEKKENEN SWCILEHNYE DIAQEFIDFI 60
YKNPTTYHVV SFFAELLDKH NFKYLSEKSN WQDSIGEDGG KFYTIRNGTN LSAFILGKNW 120
RAEKGVGVIG SHVDALTVKL KPVSFKDTAE GYGRIAVAPY GGTLNELWLD RDLGIGGRLL 180
YKKKGTNEIK SALVDSTPLP VCRIPSLAPH FGKPAEGPFD KEDQTIPVIG FPTPDEEGNE 240
PPTDDEKKSP LFGKHCIHLL RYVAKLAGVE VSELIQMDLD LFDVQKGTIG GIGKHFLFAP 300
RLDDRLCSFA AMIALICYAK DVNTEESDLF STVTLYDNEE IGSLTRQGAK GGLLESVVER 360
SSSAFTKKPV DLHTVWANSI ILSADVNHLY NPNFPEVYLK NHFPVPNVGI TLSLDPNGHM 420
ATDVVGTALV EELARRNGDK VQYFQIKNNS RSGGTIGPSL ASQTGARTID LGIAQLSMHS 480
IRAATGSKDV GLGVKFFNGF FKHWRSVYDE FGEL 514 
Gene Ontology
 GO:0000324; C:fungal-type vacuole; IDA:SGD.
 GO:0070006; F:metalloaminopeptidase activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0007039; P:vacuolar protein catabolic process; ISS:SGD. 
Interpro
 IPR001948; Peptidase_M18.
 IPR023358; Peptidase_M18_dom2. 
Pfam
 PF02127; Peptidase_M18 
SMART
  
PROSITE
  
PRINTS
 PR00932; AMINO1PTASE.