CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024756
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Piezo-type mechanosensitive ion channel component 1 
Protein Synonyms/Alias
 Protein FAM38A 
Gene Name
 Piezo1 
Gene Synonyms/Alias
 Fam38a 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
113GVTRLDLKDIFNTTRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of mechanosensitive channel required for the mechanosensitive currents. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. Overexpression in multiple cell lines generates robust mechanosensitive cation currents that are non-selective, exhibit a linear current voltage relationship, and are sensitive to ruthenium red and gadolinium. 
Sequence Annotation
 MOD_RES 1385 1385 Phosphoserine (By similarity).
 MOD_RES 1646 1646 Phosphoserine (By similarity).
 CARBOHYD 94 94 N-linked (GlcNAc...) (Potential).
 CARBOHYD 389 389 N-linked (GlcNAc...) (Potential).  
Keyword
 Cell membrane; Coiled coil; Complete proteome; Endoplasmic reticulum; Glycoprotein; Ion channel; Ion transport; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2547 AA 
Protein Sequence
MEPHVLGAGL YWLLLPCTLL AASLLRFNAL SLVYLLFLLL LPWLPGPSRH SIPGHTGRLL 60
RALLCLSLLF LVAHLAFQIC LHTVPHLDQF LGQNGSLWVK VSQHIGVTRL DLKDIFNTTR 120
LVAPDLGVLL ASSLCLGLCG RLTRKAGQSR RTQELQDDDD DDDDDDEDID AAPAVGLKGA 180
PALATKRRLW LASRFRVTAH WLLMTSGRTL VIVLLALAGI AHPSAFSSIY LVVFLAICTW 240
WSCHFPLSPL GFNTLCVMVS CFGAGHLICL YCYQTPFIQD MLPPGNIWAR LFGLKNFVDL 300
PNYSSPNALV LNTKHAWPIY VSPGILLLLY YTATSLLKLH KSCPSELRKE TPREDEEHEL 360
ELDHLEPEPQ ARDATQGEMP MTTEPDLDNC TVHVLTSQSP VRQRPVRPRL AELKEMSPLH 420
GLGHLIMDQS YVCALIAMMV WSIMYHSWLT FVLLLWACLI WTVRSRHQLA MLCSPCILLY 480
GLTLCCLRYV WAMELPELPT TLGPVSLHQL GLEHTRYPCL DLGAMLLYLL TFWLLLRQFV 540
KEKLLKKQKV PAALLEVTVA DTEPTQTQTL LRSLGELVTG IYVKYWIYVC AGMFIVVSFA 600
GRLVVYKIVY MFLFLLCLTL FQVYYTLWRK LLRVFWWLVV AYTMLVLIAV YTFQFQDFPT 660
YWRNLTGFTD EQLGDLGLEQ FSVSELFSSI LIPGFFLLAC ILQLHYFHRP FMQLTDLEHV 720
PPPGTRHPRW AHRQDAVSEA PLLEHQEEEE VFREDGQSMD GPHQATQVPE GTASKWGLVA 780
DRLLDLAASF SAVLTRIQVF VRRLLELHVF KLVALYTVWV ALKEVSVMNL LLVVLWAFAL 840
PYPRFRPMAS CLSTVWTCII IVCKMLYQLK IVNPHEYSSN CTEPFPNNTN LQPLEINQSL 900
LYRGPVDPAN WFGVRKGYPN LGYIQNHLQI LLLLVFEAVV YRRQEHYRRQ HQQAPLPAQA 960
VCADGTRQRL DQDLLSCLKY FINFFFYKFG LEICFLMAVN VIGQRMNFMV ILHGCWLVAI 1020
LTRRRREAIA RLWPNYCLFL TLFLLYQYLL CLGMPPALCI DYPWRWSKAI PMNSALIKWL 1080
YLPDFFRAPN STNLISDFLL LLCASQQWQV FSAERTEEWQ RMAGINTDHL EPLRGEPNPI 1140
PNFIHCRSYL DMLKVAVFRY LFWLVLVVVF VAGATRISIF GLGYLLACFY LLLFGTTLLQ 1200
KDTRAQLVLW DCLILYNVTV IISKNMLSLL SCVFVEQMQS NFCWVIQLFS LVCTVKGYYD 1260
PKEMMTRDRD CLLPVEEAGI IWDSICFFFL LLQRRIFLSH YFLHVSADLK ATALQASRGF 1320
ALYNAANLKS INFHRQIEEK SLAQLKRQMK RIRAKQEKYR QSQASRGQLQ SKDPQDPSQE 1380
PGPDSPGGSS PPRRQWWRPW LDHATVIHSG DYFLFESDSE EEEEALPEDP RPAAQSAFQM 1440
AYQAWVTNAQ TVLRQRRERA RQERAEQLAS GGDLNPDVEP VDVPEDEMAG RSHMMQRVLS 1500
TMQFLWVLGQ ATVDGLTRWL RAFTKHHRTM SDVLCAERYL LTQELLRVGE VRRGVLDQLY 1560
VGEDEATLSG PVETRDGPST ASSGLGAEEP LSSMTDDTSS PLSTGYNTRS GSEEIVTDAG 1620
DLQAGTSLHG SQELLANART RMRTASELLL DRRLHIPELE EAERFEAQQG RTLRLLRAGY 1680
QCVAAHSELL CYFIIILNHM VTASAASLVL PVLVFLWAML TIPRPSKRFW MTAIVFTEVM 1740
VVTKYLFQFG FFPWNSYVVL RRYENKPYFP PRILGLEKTD SYIKYDLVQL MALFFHRSQL 1800
LCYGLWDHEE DRYPKDHCRS SVKDREAKEE PEAKLESQSE TGTGHPKEPV LAGTPRDHIQ 1860
GKGSIRSKDV IQDPPEDLKP RHTRHISIRF RRRKETPGPK GTAVMETEHE EGEGKETTER 1920
KRPRHTQEKS KFRERMKAAG RRLQSFCVSL AQSFYQPLQR FFHDILHTKY RAATDVYALM 1980
FLADIVDIII IIFGFWAFGK HSAATDIASS LSDDQVPQAF LFMLLVQFGT MVIDRALYLR 2040
KTVLGKLAFQ VVLVVAIHIW MFFILPAVTE RMFSQNAVAQ LWYFVKCIYF ALSAYQIRCG 2100
YPTRILGNFL TKKYNHLNLF LFQGFRLVPF LVELRAVMDW VWTDTTLSLS NWMCVEDIYA 2160
NIFIIKCSRE TEKKYPQPKG QKKKKIVKYG MGGLIILFLI AIIWFPLLFM SLIRSVVGVV 2220
NQPIDVTVTL KLGGYEPLFT MSAQQPSIVP FTPQAYEELS QQFDPYPLAM QFISQYSPED 2280
IVTAQIEGSS GALWRISPPS RAQMKQELYN GTADITLRFT WNFQRDLAKG GTVEYTNEKH 2340
TLELAPNSTA RRQLAQLLEG RPDQSVVIPH LFPKYIRAPN GPEANPVKQL QPDEEEDYLG 2400
VRIQLRREQV GTGASGEQAG TKASDFLEWW VIELQDCKAD CNLLPMVIFS DKVSPPSLGF 2460
LAGYGIVGLY VSIVLVVGKF VRGFFSEISH SIMFEELPCV DRILKLCQDI FLVRETRELE 2520
LEEELYAKLI FLYRSPETMI KWTRERE 2547 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005261; F:cation channel activity; IMP:UniProtKB.
 GO:0008381; F:mechanically-gated ion channel activity; IDA:MGI.
 GO:0050982; P:detection of mechanical stimulus; IMP:MGI.
 GO:0033634; P:positive regulation of cell-cell adhesion mediated by integrin; ISS:UniProtKB.
 GO:0033625; P:positive regulation of integrin activation; ISS:UniProtKB.
 GO:0042391; P:regulation of membrane potential; IMP:MGI. 
Interpro
 IPR027272; Piezo. 
Pfam
 PF12166; DUF3595 
SMART
  
PROSITE
  
PRINTS