CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024648
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative high mobility group protein B1-like 1 
Protein Synonyms/Alias
 High mobility group protein B1 pseudogene 1; Putative high mobility group protein 1-like 1; HMG-1L1 
Gene Name
 HMGB1P1 
Gene Synonyms/Alias
 HMG1L1; HMGB1L1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12DPKKPRGKMSSYAFFacetylation[1, 2, 3]
12DPKKPRGKMSSYAFFubiquitination[4, 5]
50NKCSERWKTMSAKEKubiquitination[5]
55RWKTMSAKEKGKFEDubiquitination[6]
57KTMSAKEKGKFEDMAacetylation[3]
59MSAKEKGKFEDMAKAacetylation[1, 2, 3, 7]
59MSAKEKGKFEDMAKAubiquitination[4, 5]
65GKFEDMAKADKTHYEubiquitination[3, 5]
82MKTYIPPKGETKKKFacetylation[1]
90GETKKKFKDPNAPKRacetylation[3]
90GETKKKFKDPNAPKRubiquitination[5]
114SEYHPKIKGEHPGLSubiquitination[3, 4, 5, 6, 8, 9]
127LSIGDVAKKLGEMWNubiquitination[6, 10, 11]
128SIGDVAKKLGEMWNNubiquitination[5, 6, 11]
141NNTAADDKQPGEKKAubiquitination[11]
154KAAKLKEKYEKDIAAacetylation[3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 Binds preferentially single-stranded DNA and unwinds double stranded DNA (By similarity). 
Sequence Annotation
 DNA_BIND 9 79 HMG box 1.
 DNA_BIND 95 163 HMG box 2.
 MOD_RES 30 30 N6-acetyllysine (By similarity).
 MOD_RES 35 35 Phosphoserine (By similarity).
 MOD_RES 100 100 Phosphoserine (By similarity).  
Keyword
 Acetylation; Chromosome; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 211 AA 
Protein Sequence
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HSDASVNFSE FSNKCSERWK TMSAKEKGKF 60
EDMAKADKTH YERQMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYH PKIKGEHPGL 120
SIGDVAKKLG EMWNNTAADD KQPGEKKAAK LKEKYEKDIA AYQAKGKPEA AKKGVVKAEK 180
SKKKKEEEED EEDEEDEEEE DEEDEEDDDD E 211 
Gene Ontology
 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR009071; HMG_box_dom. 
Pfam
 PF09011; DUF1898
 PF00505; HMG_box 
SMART
 SM00398; HMG 
PROSITE
 PS00353; HMG_BOX_1
 PS50118; HMG_BOX_2 
PRINTS