UniProt Accession

 CPSF4_HUMAN; O95639; D6W5S8; Q6FGE6; Q86TF8; Q9BTW6 

Genbank Protein ID

 U79569; CR542161; EF191081; CH236956; CH471091; CH471091; BC003101; BC050738 

Genbank Nucleotide ID

 AAD00321.1; CAG46958.1; ABN05292.1; EAL23878.1; EAW76667.1; EAW76668.1; AAH03101.1; AAH50738.1 

Protein Name

 Cleavage and polyadenylation specificity factor subunit 4 

Protein Synonyms/Alias

 Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; NS1 effector domain-binding protein 1; Neb-1; No arches homolog 

Gene Name

 CPSF4 

Gene Synonyms/Alias

 CPSF30; NAR; NEB1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
46	AVCEFFLKAACGKGG	ubiquitination	[1]
51	FLKAACGKGGMCPFR	ubiquitination	[1]
69	GEKTVVCKHWLRGLC	ubiquitination	[1]
108	KFGECSNKECPFLHI	ubiquitination	[1]
120	LHIDPESKIKDCPWY	ubiquitination	[1]
122	IDPESKIKDCPWYDR	ubiquitination	[1]
133	WYDRGFCKHGPLCRH	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U). 

Sequence Annotation

 ZN_FING 35 61 C3H1-type 1.
 ZN_FING 62 89 C3H1-type 2.
 ZN_FING 90 117 C3H1-type 3.
 ZN_FING 118 142 C3H1-type 4.
 ZN_FING 143 169 C3H1-type 5.
 ZN_FING 243 260 CCHC-type.
 MOD_RES 202 202 Phosphoserine.
 MOD_RES 212 212 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Host-virus interaction; Metal-binding; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 269 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0046778; P:modification by virus of host mRNA processing; TAS:Reactome.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0019058; P:viral infectious cycle; TAS:Reactome. 

Interpro

 IPR000571; Znf_CCCH.
 IPR001878; Znf_CCHC. 

Pfam

 PF00642; zf-CCCH
 PF00098; zf-CCHC 

SMART

 SM00343; ZnF_C2HC
 SM00356; ZnF_C3H1 

PROSITE

 PS50103; ZF_C3H1
 PS50158; ZF_CCHC 

PRINTS