UniProt Accession

 RAD21_MOUSE; Q61550; P70219; Q3TQ09; Q810A8; Q91VB9; Q9DBU4 

Genbank Protein ID

 D49429; X98293; AF332086; AF332085; AK004746; AK163992; BC043032 

Genbank Nucleotide ID

 BAA08408.1; CAA66939.1; AAK56114.1; AAK56113.1; BAB23527.1; BAE37576.1; AAH43032.2 

Protein Name

 Double-strand-break repair protein rad21 homolog 

Protein Synonyms/Alias

 Pokeweed agglutinin-binding protein 29; PW29; SCC1 homolog 

Gene Name

 Rad21 

Gene Synonyms/Alias

 Hr21 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
358	LDLAPPTKKLMMWKE	ubiquitination	[1]
387	LWNNRLLKLFTRCLT	acetylation	[2]
387	LWNNRLLKLFTRCLT	ubiquitination	[1]
406	EDLRKRRKGGEADNL	ubiquitination	[1]
577	GLQRALAKTGAESIS	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. Plays a role in apoptosis, via its cleavage by caspase- 3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C- terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway (By similarity). The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. 

Sequence Annotation

 REGION 287 403 Interaction with WAPAL and PDS5B (By
 REGION 362 403 Interaction with STAG1 (By similarity).
 MOD_RES 46 46 Phosphoserine (By similarity).
 MOD_RES 153 153 Phosphoserine (By similarity).
 MOD_RES 175 175 Phosphoserine (By similarity).  

Keyword

 Apoptosis; Calcium; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Complete proteome; DNA damage; DNA repair; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 635 AA 

Protein Sequence

Gene Ontology

 GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
 GO:0034991; C:nuclear meiotic cohesin complex; IDA:MGI.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0071168; P:protein localization to chromatin; ISS:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara. 

Interpro

 IPR023093; Rad21/Rec8_C.
 IPR006909; Rad21/Rec8_C_eu.
 IPR006910; Rad21_Rec8_N. 

Pfam

 PF04824; Rad21_Rec8
 PF04825; Rad21_Rec8_N 

SMART

  

PROSITE

  

PRINTS